Recombinant Mouse IL-24 (NS0-expressed) Protein

Formulations:
Catalog # Availability Size / Price Qty
7807-ML-010
Product Details
Citations (2)
FAQs
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Recombinant Mouse IL-24 (NS0-expressed) Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human IL-20 R alpha and human IL-20 R beta. The ED50 for this effect is 0.07-0.35 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-24 protein
Gln66-Leu220
Accession #
N-terminal Sequence
Analysis
Gln66 predicted: No results obtained, sequencing might be blocked
Predicted Molecular Mass
18 kDa (monomer)
SDS-PAGE
19‑23 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7807-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7807-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: IL-24

Interleukin 24 (IL-24), also known as FISP (IL-4 induced secreted protein) in mouse, and mda-7 (melanoma differentiation associated gene‑7) in humans, is a member of the IL-10 family of helical cytokines (1-3). The mouse IL-24 gene encodes for a 181 amino acid (aa) precursor that includes a 26 aa signal sequence and an 18 kDa (predicted), 155 aa mature segment (4). Unlike mature human IL-24, which contains three potential N-linked glycosylation sites, mouse mature IL-24 has only one. In human, both glycosylation and the presence of an intrachain disulfide bond have been found to be necessary for full activity (5). This requirement for glycosylation is unique among IL-10 family members. Presumably, the same structural modifications apply to mouse IL-24. Mouse IL-24 is a 27 kDa protein when secreted, and is active on human receptors (1, 3). Mature mouse IL-24 shares 69%, 84%, 69%, 68% and 61% aa sequence identity with human, rat, equine, canine and bovine IL-24, respectively. A 119 aa isoform termed FISP-sp diverges at aa 77. FISP-sp has been found mainly in the endoplasmic reticulum of Th2 cells. It can dimerize with intracellular IL-24, and antagonize the pro-apoptotic effects of IL-24 (6). Under physiological conditions, cytokine-stimulated monocytes/macrophages and Th2 cells produce most secreted IL-24; other sources include B cells, keratinocytes, NK cells, and differentiating melanoma cells (1-3, 7-9). Secreted IL-24 binds and signals through complexes of IL‑20 R alpha :IL-20 R beta, or IL-22 R:IL-20 R beta (10). The IL-20 R alpha :IL-20 R beta complex is also a receptor for IL-19 and IL-20, while IL-22 R:IL-20 R beta binds IL‑20 (2, 10).  Both receptors are widely expressed, but have not been found on hematopoietic cells (3). Even so, IL-24 induces type I proinflammatory cytokines in monocytes, and inhibits plasma cell differentiation in germinal center B cells (8, 11). The phenotype of mice transgenic for IL-24 is similar to that of IL-20 and IL-22 transgenic mice, indicating overlap in their activities, particularly in the skin (12). Secreted IL-24 is anti-angiogenic, binding receptors on endothelial cells and blocking their differentiation (13). Intratumoral injection of adenovirus coding for IL-24 causes tumor-specific apoptosis in humans, but it is not clear whether secreted IL-24 contributes to this effect (14).

References
  1. Schaefer, G. et al. (2001) J. Immunol. 166:5859.
  2. Trivella, D.B.B. et al. (2010) Cell. Mol. Life Sci. 67:2909.
  3. Wang, M. and P. Liang (2005) Immunology 114:166.
  4. Swissprot Accession # Q925J3.
  5. Fuson, K.L. et al. (2009) J. Biol. Chem. 284:30526.
  6. Sahoo, A. et al. (2008) J. Biol. Chem. 283:28860.
  7. Poindexter, N.J. et al. (2005) J. Leukoc. Biol. 78:745.
  8. Maarof, G. et al. (2010) Blood 115:1718.
  9. Poindexter, N.J. et al. (2010) Exp. Dermatol. 19:714.
  10. Wang, M. et al. (2002) J. Biol Chem. 227:7341.
  11. Caudell, E.G. et al. (2002) J. Immunol. 168:6041.
  12. He, M. and P. Liang (2010) J. Immunol. 184:1793.
  13. Ramesh, R. et al. (2003) Cancer Res. 63:5105.
  14. Emdad, L. et al. (2009) Cancer Biol. Ther. 8:391.
Long Name
Interleukin 24
Entrez Gene IDs
11009 (Human); 93672 (Mouse); 170819 (Rat)
Alternate Names
C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein

Citations for Recombinant Mouse IL-24 (NS0-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Murine astrocytes produce IL-24 and are susceptible to the immunosuppressive effects of this cytokine
    Authors: AR Burmeister, MB Johnson, JJ Yaemmongko, I Marriott
    J Neuroinflammation, 2019;16(1):55.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas
    Authors: BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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