Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
983-MR-100
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Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the IL-3-induced proliferation of NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.03‑0.15 µg/mL in the presence of 2.5 µg/mL Recombinant Mouse IL‑3 R beta Fc Chimera (Catalog # 549‑R3) and 2 ng/mL of Recombinant Mouse IL‑3 (Catalog # 403‑ML).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-3 R alpha/CD123 protein
Mouse IL-3 R alpha
(Ser17-Lys331)
Accession # P26952
IEGRID Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser17
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
61.0 kDa (monomer)
SDS-PAGE
70-80 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

983-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: IL-3R alpha

The IL-3 receptor chain (IL‑3 R alpha ; also SUT-1 and CD123) is a 60‑70 kDa member of the class I cytokine receptor family of proteins (1, 2, 3). Members of this class are type I transmembrane proteins that contain two fibronectin type III (FNIII) modules and a distinctive Trp‑Ser‑x‑Trp‑Ser peptide motif in the extracellular domain. This is in contrast to class II receptors that share the same fibronectin domains but lack both the WSxWS motif and analogous cysteine spacings. Mouse IL‑3 R alpha is synthesized as a 396 amino acid (aa) precursor that contains a 16 aa signal sequence, a 315 aa extracellular region, a 24 aa transmembrane segment, and a 41 aa cytoplasmic tail (3, 4). The molecule is expressed on multiple cell types, including endothelial cells (5), monocytes (6), eosinophils (7), basophils plus mast cells (8), and plasmacytoid CD4+ T cells (9). IL‑3 R alpha serves as a low affinity binding protein for 24‑28 kDa monomeric IL‑3 (1, 3, 4, 10, 11). Following binding, the IL‑3:IL‑3 R alpha complex likely interacts with a preformed signaling homodimer comprised of either 120 kDa AIC2A (= beta IL-3) or 130 kDa AIC2B (= beta c) chains. This tetramer appears to recruit one more IL‑3:IL‑3 R alpha complex, generating a fully functional hexamer (1, 3, 4, 12, 13). Signaling is mediated by receptor‑associated cytoplasmic kinases, as class I cytokine receptors do not possess intrinsic kinase activity. Notably, the IL‑3 R complex may not exist as a singularity, but actually form higher‑order complexes with FcR gamma, beta 1 integrin, and VEGF R2 (1). There are at least two IL‑3 Ra isoform variants in mouse. One is found in the AKR and A/J strains, and shows a deletion of aa 274‑283. This is not present at the cell membrane (14). The second (called SP2) shows a deletion of aa 20‑112, generating a 50‑55 kDa membrane form that binds IL‑3, but will only signal through beta IL-3 (15, 16). IL‑3’s choice of full‑length IL‑3 R alpha (SP1) or SP2 leads to different signaling outcomes, presumably due to the engagement of different binding sites on beta IL-3. The extracellular domain of mouse IL‑3 R alpha /SP1 shares only 44% and 29% aa sequence identity with rat and human IL‑3 R alpha, respectively. Nevertheless, human IL‑3 is reported to be active on the mouse receptor complex that is expressed on select cell types (17).

References
  1. Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
  2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  3. Miyajima, A. et al. (1993) Blood 82:1960.
  4. Hara, T. & A. Miyajima (1992) EMBO J. 11:1875.
  5. Korpelainen, E.I. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11137.
  6. Ebner, S. et al. (2002) J. Immunol. 168:6199.
  7. Gregory, B. et al. (2003) J. Immunol. 170:5359.
  8. Dahl, C. et al. (2004) Allergy 59:1087.
  9. Grouard, G. et al. (1997) J. Exp. Med. 185:1101.
  10. Clark-Lewis, I. et al. (1984) J. Biol. Chem. 259:7488.
  11. Knepper, T.P. et al. (1992) Biochemistry 31:11651.
  12. Ogorochi, T. et al. (1992) Blood 79:895.
  13. Murphy, J.M. et al. (2004) J. Biol. Chem. 279:26500.
  14. Ichihara, M. et al. (1995) EMBO J. 14:939.
  15. Chen, J. et al. (2009) J. Biol. Chem. 284:5763.
  16. Mirza, S. et al. (2010) J. Biol. Chem. 285:22370.
  17. Dentelli, P. et al. (1999) J. Immunol. 163:2151.
Long Name
Interleukin 3 Receptor alpha
Entrez Gene IDs
3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)
Alternate Names
CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174

Citation for Recombinant Mouse IL-3R alpha/CD123 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interfering with multimerization of netrin-1 receptors triggers tumor cell death.
    Authors: Mille F, Llambi F, Guix C, Delloye-Bourgeois C, Guenebeaud C, Castro-Obregon S, Bredesen DE, Thibert C, Mehlen P
    Cell Death Differ., 2009;16(10):1344-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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