Recombinant Mouse Integrin alpha 3 beta 1/VLA-3 Protein, CF

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Recombinant Mouse Integrin alpha 3 beta 1/VLA-3 Protein Bioactivity
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Recombinant Mouse Integrin alpha 3 beta 1/VLA-3 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Cultrex® Mouse Laminin I (Catalog # 3400-010-01) is coated at 2 µg/mL, Recombinant alpha 3 beta 1/VLA-3 binds with an
ED50 = 150-900 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 3 beta 1/VLA-3 protein
Mouse Integrin alpha 3
Accession # Q62470-1
His-ProGGGSGGGSAcidic Tail 6-His tag
Mouse Integrin beta 1
Accession # P09055-1
His-ProGGGSGGGS Basic Tail
N-terminal Sequence
Phe33 (Integrin alpha 3) & No results obtained. Gln21 inferred from enzymatic pyroglutamate treatment revealing Thr22 (Integrin beta 1)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
116 kDa (Integrin alpha 3) & 86 kDa (Integrin beta 1)
102-163 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse Integrin alpha 3 beta 1/VLA-3 Protein Bioactivity View Larger

When Cultrex® Mouse Laminin-I (Catalog # 3400-010-01) is coated at 2 µg/mL, Recombinant Integrin a3 beta 1/VLA-3 (Catalog # 9374-A3) binds with an ED50 = 150-900 ng/mL.

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Background: Integrin alpha 3 beta 1/VLA-3

Integrin alpha 3 beta 1, also known as VLA-3 (Very Late Antigen 3), is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). It is a non-covalent heterodimer composed of two type I transmembrane glycoprotein subunits, a 130-150 kDa alpha 3 (CD49c) subunit and a 130-140 kDa beta 1 (CD29) subunit. Integrin alpha 3 only associates with the beta 1 subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 842 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). Mouse alpha 3 heavy chain is 88% aa identical to human heavy chain. Unlike the alpha 3 subunit, the mouse beta 1 subunit does not undergo proteolytic cleavage (8). The molecule contains a 708 aa extracellular region, a 23 aa TM segment, and a 47 aa cytoplasmic domain. The extracellular region contains one von Willebrand factor-like A domain and four cysteine-rich repeats. Mouse beta 1 extracellular domain shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. Integrin alpha 3 beta 1 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize it, enabling it to bind to additional factors (9).

  1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
  2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
  3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
  4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
  5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
  6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
  7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
  8. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
  9. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.
Entrez Gene IDs
3675 (Human)
Alternate Names
Integrin alpha 3 beta 1; VLA3; VLA-3

Citations for Recombinant Mouse Integrin alpha 3 beta 1/VLA-3 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family
    Authors: C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse
    PLoS ONE, 2022;17(9):e0274667.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Capture
  2. Synthetic integrin-binding immune stimulators target cancer cells and prevent tumor formation
    Authors: M Brehs, AJG Pötgens, J Steitz, K Thewes, J Schwarz, AC Conibear, M Bartneck, F Tacke, CFW Becker
    Sci Rep, 2017;7(1):17592.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance


  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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