Recombinant Mouse Integrin alpha 3 beta 1/VLA-3 Protein, CF

  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Cultrex® Mouse Laminin I (Catalog # 3400-010-01) is coated at 2 µg/mL, Recombinant alpha 3 beta 1/VLA-3 binds with an
    ED50 = 150-900 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Mouse Integrin alpha 3
    (Phe33-Glu993)
    Accession # Q62470-1
    His-ProGGGSGGGSAcidic Tail 6-His tag
    Mouse Integrin beta 1
    (Gln21-Asp728)
    Accession # P09055-1
    His-ProGGGSGGGS Basic Tail
    N-terminusC-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Phe33 (Integrin alpha 3) & No results obtained. Gln21 inferred from enzymatic pyroglutamate treatment revealing Thr22 (Integrin beta 1)
  • Structure / Form
    Noncovalently-linked heterodimer
  • Predicted Molecular Mass
    116 kDa (Integrin alpha 3) & 86 kDa (Integrin beta 1)
  • SDS-PAGE
    102-163 kDa, reducing conditions
9374-A3
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
WhenCultrex® Mouse Laminin-I (Catalog # 3400-010-01) is coated at 2 µg/mL,Recombinant Integrin alpha 3 beta 1/VLA-3 (Catalog # 9374-A3) binds with an ED50 = 150-900 ng/mL.
Background: Integrin alpha 3 beta 1/VLA-3

Integrin alpha 3 beta 1, also known as VLA-3 (Very Late Antigen 3), is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). It is a non-covalent heterodimer composed of two type I transmembrane glycoprotein subunits, a 130-150 kDa alpha 3 (CD49c) subunit and a 130-140 kDa beta 1 (CD29) subunit. Integrin alpha 3 only associates with the beta 1 subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 842 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). Mouse alpha 3 heavy chain is 88% aa identical to human heavy chain. Unlike the alpha 3 subunit, the mouse beta 1 subunit does not undergo proteolytic cleavage (8). The molecule contains a 708 aa extracellular region, a 23 aa TM segment, and a 47 aa cytoplasmic domain. The extracellular region contains one von Willebrand factor-like A domain and four cysteine-rich repeats. Mouse beta 1 extracellular domain shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. Integrin alpha 3 beta 1 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize it, enabling it to bind to additional factors (9).

  • References:
    1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
    2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
    3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
    4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
    5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
    6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
    7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
    8. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
    9. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.
  • Entrez Gene IDs:
    3675 (Human)
  • Alternate Names:
    Integrin alpha 3 beta 1; VLA3; VLA-3
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Biotinylated Antibody

WB BAM050 3  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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