Recombinant Mouse Integrin alpha 9 beta 1 Protein, CF

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Recombinant Mouse Integrin alpha 9 beta 1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. The ED50 for this effect is typically 0.6-3 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 9 beta 1 protein
Mouse Integrin alpha 9
Accession # CAC69080
Mouse Integrin beta 1
Accession # P09055
His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus
N-terminal Sequence
Tyr31 (Integrin  alpha 9) & Gln21 predicted: No results obtained, sequencing might be blocked (Integrin beta 1)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
114 kDa (Integrin  alpha 9) & 86.5 kDa (Integrin beta 1)
120-160 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Integrin alpha 9 beta 1

Integrin alpha 9 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 (CD29) subunit (1‑3). It is the non‑covalent heterodimer of 150 kDa alpha 9 and 130 kDa beta 1 type I transmembrane glycoprotein subunits (3). The alpha 9 extracellular domain (ECD) contains an N‑terminal beta ‑propeller structure, followed by domains termed thigh, calf‑1 and calf‑2 (2). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 9 beta ‑propeller to form a binding domain when the dimer is in the active, extended and open conformation. The 1006 amino acid (aa) mouse alpha 9 extracellular domain (ECD) shares 95% and 89% aa sequence identity with rat and human alpha 9, respectively, while the 708 aa mouse beta 1 ECD shares 98% aa identity with rat and 93‑94% with human, bovine, porcine, ovine, canine and feline beta 1. Each subunit has a transmembrane sequence and a short cytoplasmic tail which, for alpha 9, contains binding sites for paxillin and SSAT (spermine/spermidine-N-acetyltransferase) (4, 5). Integrin alpha 9 beta 1 inhibits cell spreading via signaling through paxillin (4). SSAT interaction with alpha 9 beta 1 allows catalysis that lifts spermine/spermidine inhibition of KIR potassium channels, and is shown to enhance cell migration or suppress airway smooth muscle contraction (5, 6). Lymphangiogenic vascular endothelia growth factors VEGF‑C and VEGF‑D are ligands for Integrin alpha 9 beta 1 (7, 8) Genetic deletion of mouse alpha 9 beta 1 results in a fatal chylothorax due to abnormal lymphatic development (9). Integrin alpha 9 beta 1 interacts in cis with G‑CSF R and enhances its signals, contributing to granulopoiesis (10). It also binds VCAM‑1, which promotes neutrophil transendothelial migration and delays apoptosis (11, 12). Other ligands for alpha 9 beta 1 include polydom/SVEP1, tenascin, osteopontin, nerve growth factor, and most members of the ADAM family (13‑16).

  1. Gupta, S.K. and N.E. Vlahakis (2010) Cell Adh. Migr. 4:194. 
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Palmer, E. L. et al. (1993) J. Cell Biol. 123:1289.
  4. Liu, S. et al. (2001) J. Biol. Chem. 276:37086.
  5. deHart, G. W. et al. (2008) Proc. Natl. Acad. Sci. USA 105:7188.
  6. Chen, C. et al. (2012) J. Clin. Invest. 122:2916.
  7. Vlahakis, N. E. et al. (2005) J. Biol. Chem. 280:4544.
  8. Vlahakis, N. E. et al. (2007) J. Biol. Chem. 282:15187.
  9. Huang, X. Z. et al. (2000) Mol. Cell. Biol. 20:5208.
  10. Chen, C. et al. (2006) Immunity 25:895.
  11. Ross, E. A. et al. (2006) Blood 107:1178.
  12. Taooka, Y. et al. (1999) J. Cell Biol. 145:413.
  13. Sato-Nishiuchi, R. et al. (2012) J. Biol. Chem. 287:25615.
  14. Marcinkiewicz, C. et al. (2000) J. Biol. Chem. 275:31930.
  15. Staniszewska, I. et al. (2008) J. Cell Sci. 121:504.
  16. Eto, K. et al. (2002) J. Biol. Chem. 277:17804.
Entrez Gene IDs
3680 (Human); 104099 (Mouse); 685004 (Rat)
Alternate Names
Integrin alpha 9 beta 1

Citation for Recombinant Mouse Integrin alpha 9 beta 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Novel ?9 Integrin Ligand, XCL1/Lymphotactin, Is Involved in the Development of Murine Models of Autoimmune Diseases
    Authors: N Matsumoto, S Kon, T Nakatsuru, T Miyashita, K Inui, K Saitoh, Y Kitai, R Muromoto, JI Kashiwakur, T Uede, T Matsuda
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay


  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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