Recombinant Mouse Integrin alpha V beta 6 Protein, CF Summary
|Mouse Integrin alpha V|
Accession # P43406
|Mouse Integrin beta 6|
Accession # Q9Z0T9
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 300 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Integrin alpha V beta 6
Integrin alpha V beta 6 is one of five alpha V integrins and the sole beta 6 integrin (1, 2). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 95 kDa beta 6 integrin subunits is expressed exclusively on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas (2‑5). The ligand interaction site of alpha V beta 6 is in the N‑terminal head region formed by an interaction of the beta 6 vWFA domain with the alpha V beta‑propeller structure (2). The alpha V subunit contains domains termed thigh, calf, and calf‑2 with a divalent cation‑binding site found at a position equivalent to the “knee”. The 958 aa mouse alpha V ECD (4), which is cleaved at aa 886 but remains associated, shares 92‑95% aa sequence identity with human and bovine alpha V, while the 687 aa mouse beta 6 ECD (5) shares 90‑96% aa sequence identity with human, rat, bovine, ovine, and porcine beta 6. Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. The beta 6 C‑terminal 11 amino acid (aa) cytoplasmic sequence transduces a signal, enhancing proliferation and inducing MMP‑9 expression (6). Either “inside‑out” signaling or Mg2+ or Mn2+ binding unfolds and activates the integrin (1). Active alpha V beta 6 binds matrix proteins fibronectin and tenascin C (2). It also binds the TGF‑ beta latency‑associated peptide (LAP) and activates TGF‑ beta 1 or TGF‑ beta 3 from large latent complexes (7). This activation requires interaction with LTBP‑1 and fibronectin, and is enhanced by PAR-1 (8, 9). Deletion of beta 6 ablates tonic inhibition of alveolar macrophages by TGF‑ beta, inhibits intestinal regulatory T cell production, and predisposes mice to inflammatory reactions in the skin, lungs, and intestines where irritations and microbial challenges are frequent (10‑12). High alpha V beta 6 expression in carcinomas may contribute to progression through its effects on TGF‑ beta and MMP activity (3). The foot-and-mouth disease virus and several other viruses can use alpha V beta 6 as a receptor, and soluble alpha V beta 6 may block virus infectivity in vitro (13, 14).
- Hynes, R.O. (2002) Cell 110:673.
- Sheppard, D. (2004) Curr. Opin. Cell Biol. 16:552.
- Bandyopadhyay, A. and S. Raghavan (2009) Curr. Drug Targets 10:645.
- Wada, J. et al. (1996) J. Cell Biol. 132:1161.
- Arend, L.J. et al. (2000) J. Am. Soc. Nephrol. 11:2297.
- Dixit, R.B. et al. (1996) J. Biol. Chem. 271:25976.
- Munger, J.S. et al. (1999) Cell 96:319.
- Fontana, L. et al. (2005) FASEB J. 19:1798.
- Jenkins, R.G. et al. (2006) J. Clin. Invest. 116:1606.
- Huang, X.Z. et al. (1996) J. Cell Biol. 133:921.
- Morris, D.G. et al. (2003) Nature 422:169.
- Chen, X. et al. (2011) J. Leukoc. Biol. 90:751.
- Berryman, S. et al. (2005) J. Virol. 79:8519.
- Heikkila, O. et al. (2009) J. Gen. Virol. 90:197.
Citation for Recombinant Mouse Integrin alpha V beta 6 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection
Authors: M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi
PloS Pathogens, 2021;17(12):e1010083.
Sample Types: Whole Cells
What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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