Recombinant Mouse Integrin alpha V beta 6 Protein, CF

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Recombinant Mouse Integrin alpha V beta 6 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Integrin  alpha V beta 6 at 2.0 µg/mL can bind Recombinant Human LAP TGF‑ beta 1 (Catalog # 246-LP) with an apparent KD <0.1 nM.
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha V beta 6 protein
Mouse Integrin alpha V
Accession # P43406


Acidic Tail HHHHHH
Mouse Integrin beta 6
Accession # Q9Z0T9


Basic Tail
N-terminal Sequence
Phe31 ( alpha V subunit) & Gly22 ( beta 6 subunit)
Structure / Form
Non-covalently-linked heterodimer
Predicted Molecular Mass
115 kDa ( alpha V subunit) & 82.6 kDa ( beta 6 subunit)
135-150 kDa & 110-125 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Integrin alpha V beta 6

Integrin alpha V beta 6 is one of five alpha V integrins and the sole beta 6 integrin (1, 2). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 95 kDa beta 6 integrin subunits is expressed exclusively on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas (2‑5). The ligand interaction site of alpha V beta 6 is in the N‑terminal head region formed by an interaction of the beta 6 vWFA domain with the alpha V beta‑propeller structure (2). The alpha V subunit contains domains termed thigh, calf, and calf‑2 with a divalent cation‑binding site found at a position equivalent to the “knee”. The 958 aa mouse  alpha V ECD (4), which is cleaved at aa 886 but remains associated, shares 92‑95% aa sequence identity with human and bovine alpha V, while the 687 aa mouse beta 6 ECD (5) shares 90‑96% aa sequence identity with human, rat, bovine, ovine, and porcine  beta 6. Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. The beta 6 C‑terminal 11 amino acid (aa) cytoplasmic sequence transduces a signal, enhancing proliferation and inducing MMP‑9 expression (6). Either “inside‑out” signaling or Mg2+ or Mn2+ binding unfolds and activates the integrin (1). Active alpha V beta 6 binds matrix proteins fibronectin and tenascin C (2). It also binds the TGF‑ beta latency‑associated peptide (LAP) and activates TGF‑ beta 1 or TGF‑ beta 3 from large latent complexes (7). This activation requires interaction with LTBP‑1 and fibronectin, and is enhanced by PAR-1 (8, 9). Deletion of beta 6 ablates tonic inhibition of alveolar macrophages by TGF‑ beta, inhibits intestinal regulatory T cell production, and predisposes mice to inflammatory reactions in the skin, lungs, and intestines where irritations and microbial challenges are frequent (10‑12). High alpha V beta 6 expression in carcinomas may contribute to progression through its effects on TGF‑ beta and MMP activity (3). The foot-and-mouth disease virus and several other viruses can use alpha V beta 6 as a receptor, and soluble alpha V beta 6 may block virus infectivity in vitro (13, 14).

  1. Hynes, R.O. (2002) Cell 110:673.
  2. Sheppard, D. (2004) Curr. Opin. Cell Biol. 16:552.
  3. Bandyopadhyay, A. and S. Raghavan (2009) Curr. Drug Targets 10:645.
  4. Wada, J. et al. (1996) J. Cell Biol. 132:1161.
  5. Arend, L.J. et al. (2000) J. Am. Soc. Nephrol. 11:2297.
  6. Dixit, R.B. et al. (1996) J. Biol. Chem. 271:25976.
  7. Munger, J.S. et al. (1999) Cell 96:319.
  8. Fontana, L. et al. (2005) FASEB J. 19:1798.
  9. Jenkins, R.G. et al. (2006) J. Clin. Invest. 116:1606.
  10. Huang, X.Z. et al. (1996) J. Cell Biol. 133:921.
  11. Morris, D.G. et al. (2003) Nature 422:169.
  12. Chen, X. et al. (2011) J. Leukoc. Biol. 90:751.
  13. Berryman, S. et al. (2005) J. Virol. 79:8519.
  14. Heikkila, O. et al. (2009) J. Gen. Virol. 90:197.
Entrez Gene IDs
3685 (Human)
Alternate Names
CD51; Integrin alpha V beta 6; integrin subunit alpha V; ITGAV; MSK8; VNRA; VTNR

Citation for Recombinant Mouse Integrin alpha V beta 6 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The RGD-binding integrins alphavbeta6 and alphavbeta8 are receptors for mouse adenovirus-1 and -3 infection
    Authors: M Bieri, R Hendrickx, M Bauer, B Yu, T Jetzer, B Dreier, PRE Mittl, J Sobek, A Plückthun, UF Greber, S Hemmi
    PloS Pathogens, 2021;17(12):e1010083.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay


  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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