Recombinant Mouse L-Selectin/CD62L His-tag Protein, CF

Catalog # Availability Size / Price Qty
10072-LS-100
Recombinant Mouse L-Selectin/CD62L His-tag Protein, CF SDS-PAGE
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Recombinant Mouse L-Selectin/CD62L His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse L‑Selectin/CD62L is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse MAdCAM‑1 Fc Chimera (Catalog # 993-MC) that produces 50% of the optimal binding response is 2-12 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse L-Selectin/CD62L protein
Trp39-Asn332, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Trp39
Predicted Molecular Mass
34 kDa
SDS-PAGE
62-83 kDa, reducing conditions

Product Datasheets

Data Image

SDS-PAGE Recombinant Mouse L-Selectin/CD62L His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse L‑Selectin/CD62L was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 62-83 kDa.

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Background: L-Selectin/CD62L

L-Selectin (also known as Leukocyte Selectin, LAM‑1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, and CD62L), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90 - 100 kDa. Human and mouse L-Selectin share 76% amino acid sequence homology.

L-Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction involving ICAM-1 and VCAM-1 leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble L-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Entrez Gene IDs
6402 (Human); 20343 (Mouse); 29259 (Rat)
Alternate Names
CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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