Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF

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993-MC-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (10)
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Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 x 104 cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 80-90% will adhere after MnCl2 stimulation for 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse MAdCAM-1 protein
Mouse MAdCAM-1
(Gln22 - Thr365)
Accession # NP_038619
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln22 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
63 kDa (monomer)
SDS-PAGE
85-95 kDa, reducing conditions

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993-MC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

993-MC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha 4 beta 7, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha 4 beta 7 binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha 4 beta 7-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha 4 beta 7 interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.

References
  1. Briskin, M.J. et al. (1993) Nature 363:461.
  2. Yang, X.D. et al. (1997) Diabetes 46:1542.
  3. Sampaio, S.O. et al. (1995) J. Immunol. 155:2477.
  4. Kraal, G. et al. (1995) Am. J. Pathol. 147:763.
  5. Berg, E.L. et al. (1993) Nature 366:695.
  6. Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.
Long Name
Mucosal Addressin Cell Adhesion Molecule 1
Entrez Gene IDs
8174 (Human); 17123 (Mouse)
Alternate Names
hMAdCAM-1; MACAM1; MAdCAM1; MAdCAM-1; mucosal addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule-1; mucosal vascular addressin cell adhesion molecule 1

Citations for Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. A stem cell reporter based platform to identify and target drug resistant stem cells in myeloid leukemia
    Authors: K Spinler, J Bajaj, T Ito, B Zimdahl, M Hamilton, A Ahmadi, CS Koechlein, N Lytle, HY Kwon, F Anower-E-K, H Sun, A Blevins, J Weeks, M Kritzik, J Karlseder, MH Ginsberg, PW Park, JD Esko, T Reya
    Nat Commun, 2020;11(1):5998.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking
    Authors: T Savinko, C Guenther, LM Uotila, M Llort Asen, S Yao, S Tojkander, SC Fagerholm
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Differential effects of ?4?7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo
    Gut, 2016;65(10):1642-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Pretreatment of Mesenchymal Stem Cells Manipulates Their Vasculoprotective Potential While Not Altering Their Homing Within the Injured Gut.
    Authors: Kavanagh D, Suresh S, Newsome P, Frampton J, Kalia N
    Stem Cells, 2015;33(9):2785-97.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness.
    Authors: Wang X, Rodda L, Bannard O, Cyster J
    J Immunol, 2014;192(10):4601-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Multifunctional activity of a small tellurium redox immunomodulator compound, AS101, on dextran sodium sulfate-induced murine colitis.
    Authors: Halpert G, Eitan T, Voronov E, Apte R, Rath-Wolfson L, Albeck M, Kalechman Y, Sredni B
    J Biol Chem, 2014;289(24):17215-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. TLR activation excludes circulating naive CD8+ T cells from gut-associated lymphoid organs in mice.
    Authors: Heidegger S, Kirchner S, Stephan N, Bohn B, Suhartha N, Hotz C, Anz D, Sandholzer N, Stecher B, Russmann H, Endres S, Bourquin C
    J Immunol, 2013;190(10):5313-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  8. Difference in Th1 and Th17 lymphocyte adhesion to endothelium.
    Authors: Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH
    J. Immunol., 2012;188(3):1421-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
    Authors: Arthos J, Cicala C, Martinelli E, MacLeod K, Van Ryk D, Wei D, Xiao Z, Veenstra TD, Conrad TP, Lempicki RA, McLaughlin S, Pascuccio M, Gopaul R, McNally J, Cruz CC, Censoplano N, Chung E, Reitano KN, Kottilil S, Goode DJ, Fauci AS
    Nat. Immunol., 2008;9(3):301-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Aberrant activation of integrin alpha4beta7 suppresses lymphocyte migration to the gut.
    Authors: Park EJ, Mora JR, Carman CV, Chen J, Sasaki Y, Cheng G, von Andrian UH, Shimaoka M
    J. Clin. Invest., 2007;117(9):2526-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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