Recombinant Mouse LRP-6 His Tagged Protein, CF

Catalog # Availability Size / Price Qty
2960-LR-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Mouse LRP-6 His Tagged Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. rmLRP-6 binds rhDkk-1, biotin with an apparent KD <15 nM. Biotinylated rmWnt-3a immobilized on a streptavidin-coated plate at 1 µg/mL can bind rmLRP-6 with a linear range of 0.2-10 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse LRP-6 protein
Ala20-Pro1368, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala20
Predicted Molecular Mass
152.6 kDa
SDS-PAGE
170 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2960-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: LRP-6

The low-density lipoprotein (LDL) receptor-related protein 5 (LRP-5) and LRP-6 constitute a distinct subgroup of the LDL receptor family (1). Both LRP-5 and LRP-6 are type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins (FZD) (2, 3, 4). The mouse LRP-6 cDNA encodes a 1613 amino acid (aa) residue precursor including a 19 aa signal sequence, 1351 aa extracellular domain (ECD), a 23 aa transmembrane (TM) segment, and a 20 aa cytoplasmic domain (5). The ECD contains 20 YWTD motif-containing LDLR-B domains, fourEGF-like repeats, and three cysteine-rich LDLR-A repeats. The ECD of mouse LRP-6 shares 71% aa sequence identity with the ECD of mouse LRP-5 and 98% aa sequence identity with the ECD of human, rat, and canine LRP-6. The intracellular region of LRP-6 contains repeated PPPSP motifs. When the Ser/Thr in these motifs are phosphorylated, LRP-6 can interact with Axin and propagate canonical Wnt signal transduction (6, 7). LRP-6 forms inactive homodimers via its YWTD-EGF domains (8). Wnt binding to FZD and subsequent association with LRP-6 lead to activating conformational changes in LRP-6 cytoplasmic domains (8). LRP-6 can also interact directly with the Dickkopf (Dkk), sclerostin, and Wise proteins which are modulators of Wnt signaling (9 - 14). Formation of a ternary complex of LRP-6, Dkk-1, and Kremen triggers the internalization of the complex and removal LRP-6 from the cell surface. LRP-5 and LRP-6 share overlapping functions in diverse embryonic developmental processes (15).

References
  1. Schneider, W.J. and J. Nimpf (2003) Cell Mol Life Sci. 60:892.
  2. He, X. et al. (2004) Development 131:1663.
  3. Seto, E.S. and H.J. Bellen (2004) Trends Cell Biol. 14:45. 
  4. Pinson, K.I. et al. (2000) Nature 407:535. 
  5. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
  6. Tamai, K. et al. (2000) Nature 407:530. 
  7. Tamai, K. et al. (2004) Mol. Cell 13:149. 
  8. Liu, G. et al. (2003) Mol. Cell. Biol. 23:5825.
  9. Mao, B. et al. (2001) Nature 411:321.
  10. Semenov, M.V. et al. (2001) Curr. Biol. 11:951.
  11. Bafico, A. et al. (2001) Nat. Cell Biol. 3:683.
  12. Li, X. et al. (2005) J. Biol. Chem. 280:19883.
  13. Semenov, M. et al. (2005) J. Biol. Chem. 280:26770.
  14. Itasaki, N. et al. (2003) Development 130:4295.
  15. Kelly, O. et al. (2004) Development 131:2803.
Long Name
LDL Receptor-related Protein 6
Entrez Gene IDs
4040 (Human); 16974 (Mouse)
Alternate Names
ADCAD2; FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; low-density lipoprotein receptor-related protein 6; LRP6; LRP-6

Citations for Recombinant Mouse LRP-6 His Tagged Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy
    Authors: R Chidiac, M Abedin, G Macleod, A Yang, PE Thibeault, LL Blazer, JJ Adams, L Zhang, H Roehrich, HN Jo, S Seshagiri, SS Sidhu, HJ Junge, S Angers
    Embo Molecular Medicine, 2021;0(0):e13977.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  2. CCN2 modulates hair follicle cycling in mice.
    Authors: Liu S, Leask A
    Mol Biol Cell, 2013;24(24):3939-44.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  3. Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways.
    Authors: Krause C, Korchynskyi O, de Rooij K, Weidauer SE, de Gorter DJ, van Bezooijen RL, Hatsell S, Economides AN, Mueller TD, Lowik CW, ten Dijke P
    J. Biol. Chem., 2010;285(53):41614-26.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

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