Recombinant Mouse Matrilin-4 Protein, CF

Catalog # Availability Size / Price Qty
3380-MN-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Matrilin-4 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhCOMP/his (2 µg/mL, 100 µL/well) can bind rmMatrilin-4 with a linear range of 3-200 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Matrilin-4 protein
Gln22-Lys624, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln22 predicted
Predicted Molecular Mass
67.2 kDa
SDS-PAGE
75 kDa, reducing conditions

Product Datasheets

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3380-MN

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3380-MN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Matrilin-4

Matrilin-4 is a 73 kDa secreted glycoprotein that is a member of the matrilin family of the von Willebrand Factor-A (vWA) domain-containing superfamily (1). Matrilins are modular extracellular matrix proteins that serve as adaptors and linkers for other matrix proteins. Matrilin-4, like Matrilin-2, has a broad distribution in both cartilage and in loose connective tissue such as dermis, lung and kidney, while matrilins 1 and 3 are limited to cartilage. Matrilin-4 is present in nervous tissue and is abundant in the brain (2, 3). Mature mouse Matrilin-4 shares 98%, 90%, 89% and 66% amino acid (aa) identity with rat, human, canine and chicken Matrilin-4, respectively. The 624 aa mouse Matrilin-4 contains a 22 aa signal sequence, two potential glycosylation sites, and four cysteine-rich EGF-like domains placed between two vWA domains. A short isoform lacks the N-terminal vWA domain (aa 28 - 217). A C-terminal alpha -helix/coiled-coil region (aa 590 - 623) by which multimers are formed is often proteolytically removed so that Matrilin-4 is found as a mixture of monomers with homo- or hetero- dimers and trimers. Matrilin-4 forms multimers with Matrilins 1 and 2 but not with Matrilin-3 (3, 4). The N-terminal vWA domains of Matrilins associate with collagen IV microfibrils via the proteoglycans biglycan and decorin, linking the fibrils with other matrix constituents aggrecan and collagen II (5, 6). Matrilins also show calcium-dependent binding to the cartilage oligomeric matrix protein (COMP); this interaction is of high affinity for oligomeric Matrilin-4 and somewhat lower affinity for monomeric Matrilin-4 (6). Functions and distributions of Matrilins overlap enough so that knockouts of Matrilins 1, 2 and 3 lack obvious phenotypes (1).

References
  1. Wagener, R. et al. (2005) FEBS Lett. 579:3323.
  2. Klatt, A.R. et al. (2002) Matrix Biol. 21:289.
  3. Klatt, A.R. et al. (2001) J. Biol. Chem. 276:17267.
  4. Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
  5. Wiberg, C. et al. (2003) J. Biol. Chem. 278:37698.
  6. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
  7. Frank, S. et al. (2002) J. Biol. Chem. 277:19071.
Entrez Gene IDs
8785 (Human); 17183 (Mouse); 296358 (Rat)
Alternate Names
FLJ14417; MATN4; matrilin 4; Matrilin4; Matrilin-4

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