Recombinant Mouse Midkine Protein, CF Summary
Lys23-Asp140, with an N-terminal Met
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Midkine (MK), also known as Neurite Growth Promoting Factor 2 (NEGF2), belongs to a family of neurotrophic and developmentally-regulated heparin-binding molecules consisting of MK and pleiotrophin (1). MK is a highly basic, non-glycosylated polypeptide consisting of two domains stabilized by five intrachain disulfide bonds (2). Mature mouse MK is 118 amino acids (aa) in length, approximately 13 kDa, and shares 85% and 95% aa sequence identity with the human and rat protein, respectively. MK was originally identified as being over-expressed during embryogenesis but having minimal expression in adult tissue (3). While early evidence suggested MK promoted neurite outgrowth (4), MK has since been implicated in diverse biological processes ranging from angiogenesis and neurogenesis to inflammation and disease (5, 6). Depending on the function, MK signals through a wide range of varied receptors from Receptor-like Protein Tyrosine Phosphatase beta (RPTP-beta ) to syndecans to Lipoprotein receptor-related proteins (Lrp1) (5, 6). MK may play a significant role in tumorigenesis as over-expression has been observed in many cancers and research has focused on the utility of using MK as a biomarker for cancer and other diseases (5, 7, 8). The presence of MK in the senile plaques of patients with Alzheimer's disease is believed to suppress progression of the disease (9), while MK over-expression in pancreatic or breast cancer is associated with poor prognosis (7, 10, 11).
- Bohlen, P. and I. Kovesdi (1991) Prog. Growth Factor Res. 3:143.
- Fabri L et al. (1993) J Chromatogr. 646:213.
- Muramatsu, T. (1993) Int. J. Dev. Biol. 37:183.
- Asai, T. et al. (1997) Biochem. Biophys. Res. Commun. 236:66.
- Muramatsu, T. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86:410.
- Sorrelle N et al. (2017) J Leukoc Biol. 102:277.
- Wellstein, A. (2012) Front Oncol. 2:192.
- Kadomatsu K and Muramatsu T (2004) Cancer Lett. 204:127.
- Salama RH et al. (2005) Prog Neuropsychopharmacol Biol Psychiatry. 29:611.
- Jono H and Ando Y (2010) Cancers 2:624.
- Ibusuki M et al (2009) Cancer Sci. 100:1735.
Citation for Recombinant Mouse Midkine Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia
Authors: J Chatterjee, S Sanapala, O Cobb, A Bewley, AK Goldstein, E Cordell, X Ge, JR Garbow, MJ Holtzman, DH Gutmann
Nature Communications, 2021;12(1):7122.
Sample Types: Whole Cells
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