Recombinant Mouse Midkine Protein, CF

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Recombinant Mouse Midkine Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Midkine, immobilized at 125-250 ng/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
E. coli-derived mouse Midkine protein
Lys23-Asp140, with an N-terminal Met
Accession #
N-terminal Sequence
Predicted Molecular Mass
13 kDa
15 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Midkine

Midkine (MK), also known as Neurite Growth Promoting Factor 2 (NEGF2), belongs to a family of neurotrophic and developmentally-regulated heparin-binding molecules consisting of MK and pleiotrophin (1). MK is a highly basic, non-glycosylated polypeptide consisting of two domains stabilized by five intrachain disulfide bonds (2). Mature mouse MK is 118 amino acids (aa) in length, approximately 13 kDa, and shares 85% and 95% aa sequence identity with the human and rat protein, respectively. MK was originally identified as being over-expressed during embryogenesis but having minimal expression in adult tissue (3). While early evidence suggested MK promoted neurite outgrowth (4), MK has since been implicated in diverse biological processes ranging from angiogenesis and neurogenesis to inflammation and disease (5, 6). Depending on the function, MK signals through a wide range of varied receptors from Receptor-like Protein Tyrosine Phosphatase beta (RPTP-beta ) to syndecans to Lipoprotein receptor-related proteins (Lrp1) (5, 6). MK may play a significant role in tumorigenesis as over-expression has been observed in many cancers and research has focused on the utility of using MK as a biomarker for cancer and other diseases (5, 7, 8). The presence of MK in the senile plaques of patients with Alzheimer's disease is believed to suppress progression of the disease (9), while MK over-expression in pancreatic or breast cancer is associated with poor prognosis (7, 10, 11).

  1. Bohlen, P. and I. Kovesdi (1991) Prog. Growth Factor Res. 3:143.
  2. Fabri L et al. (1993) J Chromatogr. 646:213.
  3. Muramatsu, T. (1993) Int. J. Dev. Biol. 37:183.
  4. Asai, T. et al. (1997) Biochem. Biophys. Res. Commun. 236:66.
  5. Muramatsu, T. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86:410.
  6. Sorrelle N et al. (2017) J Leukoc Biol. 102:277.
  7. Wellstein, A. (2012) Front Oncol. 2:192.
  8. Kadomatsu K and Muramatsu T (2004) Cancer Lett. 204:127.
  9. Salama RH et al. (2005) Prog Neuropsychopharmacol Biol Psychiatry. 29:611.
  10. Jono H and Ando Y (2010) Cancers 2:624.
  11. Ibusuki M et al (2009) Cancer Sci. 100:1735.
Entrez Gene IDs
4192 (Human); 17242 (Mouse)
Alternate Names
Amphiregulin-associated protein; ARAP; MDK; MEK; Midgestation and kidney protein; midkine (neurite growth-promoting factor 2); Midkine; MK1; MKARAP; NEGF2; NEGF2FLJ27379; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein

Citations for Recombinant Mouse Midkine Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Neuronal hyperexcitability drives central and peripheral nervous system tumor progression in models of neurofibromatosis-1
    Authors: C Anastasaki, J Mo, JK Chen, J Chatterjee, Y Pan, SM Scheaffer, O Cobb, M Monje, LQ Le, DH Gutmann
    Nature Communications, 2022-05-19;13(1):2785.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia
    Authors: J Chatterjee, S Sanapala, O Cobb, A Bewley, AK Goldstein, E Cordell, X Ge, JR Garbow, MJ Holtzman, DH Gutmann
    Nature Communications, 2021-12-08;12(1):7122.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay


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