Recombinant Mouse Midkine Protein, CF

R&D Systems | Catalog # 9760-MD

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Mouse Midkine Protein (9760-MD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P12025

Applications

Bioactivity
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Product Specifications

Source

E. coli-derived mouse Midkine protein
Lys23-Asp140, with an N-terminal Met

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met 

Predicted Molecular Mass

13 kDa

SDS-PAGE

15 kDa, reducing conditions

Activity

Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.
Recombinant Mouse Midkine, immobilized at 125-250 ng/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.

Formulation, Preparation, and Storage

9760-MD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Midkine

Midkine (MK), also known as Neurite Growth Promoting Factor 2 (NEGF2), belongs to a family of neurotrophic and developmentally-regulated heparin-binding molecules consisting of MK and pleiotrophin (1). MK is a highly basic, non-glycosylated polypeptide consisting of two domains stabilized by five intrachain disulfide bonds (2). Mature mouse MK is 118 amino acids (aa) in length, approximately 13 kDa, and shares 85% and 95% aa sequence identity with the human and rat protein, respectively. MK was originally identified as being over-expressed during embryogenesis but having minimal expression in adult tissue (3). While early evidence suggested MK promoted neurite outgrowth (4), MK has since been implicated in diverse biological processes ranging from angiogenesis and neurogenesis to inflammation and disease (5, 6). Depending on the function, MK signals through a wide range of varied receptors from Receptor-like Protein Tyrosine Phosphatase beta (RPTP-beta ) to syndecans to Lipoprotein receptor-related proteins (Lrp1) (5, 6). MK may play a significant role in tumorigenesis as over-expression has been observed in many cancers and research has focused on the utility of using MK as a biomarker for cancer and other diseases (5, 7, 8). The presence of MK in the senile plaques of patients with Alzheimer's disease is believed to suppress progression of the disease (9), while MK over-expression in pancreatic or breast cancer is associated with poor prognosis (7, 10, 11).

References

  1. Bohlen, P. and I. Kovesdi (1991) Prog. Growth Factor Res. 3:143.
  2. Fabri L et al. (1993) J Chromatogr. 646:213.
  3. Muramatsu, T. (1993) Int. J. Dev. Biol. 37:183.
  4. Asai, T. et al. (1997) Biochem. Biophys. Res. Commun. 236:66.
  5. Muramatsu, T. (2010) Proc Jpn Acad Ser B Phys Biol Sci 86:410.
  6. Sorrelle N et al. (2017) J Leukoc Biol. 102:277.
  7. Wellstein, A. (2012) Front Oncol. 2:192.
  8. Kadomatsu K and Muramatsu T (2004) Cancer Lett. 204:127.
  9. Salama RH et al. (2005) Prog Neuropsychopharmacol Biol Psychiatry. 29:611.
  10. Jono H and Ando Y (2010) Cancers 2:624.
  11. Ibusuki M et al (2009) Cancer Sci. 100:1735.

Alternate Names

ARAP, MDK, MEK, MK1, NEGF2

Entrez Gene IDs

4192 (Human); 17242 (Mouse)

Gene Symbol

MDK

UniProt

P12025 (Mouse)

Additional Midkine Products

Product Documents for Recombinant Mouse Midkine Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Midkine Protein, CF

For research use only

Citations for Recombinant Mouse Midkine Protein, CF

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