Recombinant Mouse Mimecan Protein, CF Summary
Ala20-Phe298, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in PBS.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mimecan is a 34 kDa secreted monomeric glycoprotein that belongs to the SLRP, or small leucine-rich proteoglycan family of matrix molecules (1, 2). All SLRP family members contain multiple leucine rich repeats, of which there are two types; a 21 amino acid (aa) proline-containing S-type, and a 26 aa phenylalanine-containing T-type (2, 3). Mimecan is a class III SLRP subfamily member, which means it contains an S-T (vs. S-T-T) repeating motif. Mouse Mimecan is synthesized as a 298 aa precursor that contains a 19 aa signal sequence and a 279 aa mature region (3, 4). The mature region contains a 75 aa N-terminus followed by a 13 aa cysteine-rich region and seven consecutive LRR repeats. There is one (presumably occupied) C-terminal N-linked glycosylation site and a potential keratan sulfate attachment point in LRR #4 (aa 161 - 180) (3). Nomenclature for the mimecan molecule is confusing. Mimecan is best thought of as being the full-length, 279 aa glycoprotein (5). Proteolytic removal of the first 56 aa by BMP-1/Tolloid-like proteinases generates the 25 kDa KSPG form (aa 57 - 279) (3). The 17 kDa, 56 aa mature N-terminus has been referred to as the minecam prosegment (3). When the prosegment is removed, mature mouse KSPG (aa 57 - 279) is 98%, 91%, 90% and 91% aa identical to rat, bovine, human and canine KSPG, respectively. A 12 kDa form (aa 175 - 279) consisting of the 105 aa of the C-terminus (OIF/osteoglycan) has also been observed (6). Noncoding alternate splice forms for mouse are believed to exist and should be expressed in a tissue specific fashion (7, 8). No splice forms are known that impact the coding region. Mimecan seems to exist as proteoglycan (PG) and non-PG forms. In cornea, keratan sulfate is present, although the exact function of the PG is unknown. The corneal extracellular matrix is normally transparent. While mimecan is reported to inhibit fibrillogenesis, possibly by limiting lateral accretion of collagen monomers, it is unclear if the mimican PG actually contributes to transparency (3, 9). In other tissues, mimecan and its derivatives can exist as simple glycoproteins, absent of any PG adduct (6). The uncertain nature of mimecan’s structure and function gives rise to its name: Mime is a Norse-legend dwarf that was notoriously deceitful and misleading (6).
- Iozzo, R.V. (1999) J. Biol. Chem. 275:18843.
- Matsushima, N. et al. (2000) Proteins 38:210.
- Ge, G. et al. (2004) J. Biol. Chem. 279:41626.
- Ujita, M. et al. (1995) Gene 158:237.
- Hu, S-M. et al. (2005) J. Clin. Endocrinol. Metab. 90:6657
- Funderburgh, J.L. et al. (1997) J. Biol. Chem. 272:28089.
- Tasheva, E.S. et al. (1999) J. Biol. Chem. 274:18693.
- Tasheva, E.S. et al. (1997) J. Biol. Chem. 272:32551.
- Tasheva, E.S. et al. (2002) Mol. Vis. 8:407.
Citations for Recombinant Mouse Mimecan Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases
Authors: AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano
Sci Rep, 2020;10(1):12912.
Species: Mouse, Snake
Sample Types: Protein, Recombinant Protein
Osteoglycin, a novel coordinator of bone and glucose homeostasis
Authors: NJ Lee, N Ali, L Zhang, Y Qi, I Clarke, RF Enriquez, M Brzozowska, IC Lee, MJ Rogers, DR Laybutt, JR Center, PA Baldock, H Herzog
Mol Metab, 2018;13(0):30-44.
Sample Types: In Vivo
Applications: In Vivo
Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases.
Authors: Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G
Invest Ophthalmol Vis Sci, 2013;54(2):1014-25.
Sample Types: Protein
Applications: Enzyme Assay
Effects of Ultraviolet-A and Riboflavin on the Interaction of Collagen and Proteoglycans during Corneal Cross-linking.
Authors: Zhang Y, Conrad AH, Conrad GW
J. Biol. Chem., 2011;286(15):13011-22.
Sample Types: Whole Cells
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