Recombinant Mouse Neogenin Fc Chimera Protein, CF Summary
Accession # NP_032710
|IEGRID||Human IgG1 |
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Neogenin is a type I transmembrane protein belonging to the Ig superfamily. It is composed of an extracellular segment containing four Ig-like C2-type domains and six Fibronectin type III domains (1). Neogenin has a molecular weight of approximately 190 kDa, and the extracellular domain of the mouse protein shares 91% and 94% amino acid sequence identity with the human and rat orthologs, respectively (1). Five different isoforms are produced from alternative splicing of mouse Neo1. Neogenin is widely expressed in both neuronal and non-neuronal tissues of the developing mouse embryo, and in most tissues in adult mice (2). It is a multifunctional cell-surface receptor that binds to members of the Netrin, Repulsive Guidance Molecule (RGM) and Bone Morphogenetic Protein (BMP) families (3-5). It has also been shown to interact with members of the UNC5 family and in certain instances, associate with CDO as a co-receptor (6-8). Neogenin appears to be involved in the regulation of multiple developmental processes including development of the central nervous system (CNS), myogenesis, angiogenesis, and formation of mammary glands (4, 5, 7-9). During CNS development, Neogenin regulates neural tube closure, neuronal differentiation, and cell survival (4, 5, 7). It also mediates
Netrin-1-dependent attraction and RGM-A-dependent repulsion of growing axons (4, 5, 7, 10). Additionally, Neogenin binding to RGM and Netrin proteins regulates cell-cell adhesion, cell migration, tissue organization, and adult neurogenesis (4, 7, 11). Neogenin is thought to be involved in tumorgenesis and cancer cell invasiveness in brain and gastric cancers (12-14).
- Meyerhardt, J.A. et al. (1997) Oncogene 14:1129.
- Keeling, S.L. et al. (1997) Oncogene 15:691.
- Hagihara, M. et al. (2011) J. Biol. Chem. 286:5157.
- Tian, C. and J. Liu (2013) Mol. Reprod. Dev. 80:700.
- Severyn, C.J. et al. (2009) Biochem. J. 422:393.
- van den Heuvel, D.M. et al. (2013) PLoS ONE 8:e55828.
- De Vries, M. and H.M. Cooper (2008) J. Neurochem. 106:1483.
- Krauss, R.S. et al. (2005) J. Cell Sci. 118:2355.
- Srinivasan, K. et al. (2003) Dev. Cell 4:371.
- de Castro, F. (2003) News Physiol. Sci. 18:130.
- O’Leary, C.J. et al. (2015) Stem Cells 33:503.
- Milla, L.A. et al. (2014) Int. J. Cancer 134:21.
- Akino, T. et al. (2014) Cancer Res. 74:3716.
- Kim, S.J. et al. (2014) Oncotarget 5:3386.
Citation for Recombinant Mouse Neogenin Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B.
Authors: Lejmi E, Leconte L, Pedron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouet J
Proc. Natl. Acad. Sci. U.S.A., 2008;105(34):12491-6.
Sample Types: Recombinant Protein
Applications: ELISA (Capture)
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