Recombinant Mouse Neurocan Protein, CF

Catalog # Availability Size / Price Qty
5800-NC-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Recombinant Mouse Neurocan Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.

When 5 x 104 cells/well are added to mouse Neurocan coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is
0.1‑0.4 μg/mL.

Source
Chinese Hamster Ovary cell line, CHO-derived mouse Neurocan protein
Asp23-Asp637, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Asp23
Predicted Molecular Mass
67.1 kDa
SDS-PAGE
120-200 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5800-NC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Neurocan

Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37 - 157), two Link domains (aa 159 - 254 and 258 - 356), two EGF-like domains (aa 960 - 996 and 998 - 1034), one C-type lectin-like domain (aa 1036 - 1165), one Sushi domain (aa 1165 - 1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2 - 3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).

References
  1. Inatani, M. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2350.
  2. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
  3. Retzler, C. et al. (1996) J. Biol. Chem. 271:27304.
  4. Quaglia, X. et al. (2008) Brain 131:240.
Entrez Gene IDs
1463 (Human); 13004 (Mouse); 58982 (Rat)
Alternate Names
chondroitin sulfate proteoglycan 3 (neurocan); Chondroitin sulfate proteoglycan 3; CSPG3; CSPG3neurocan core protein; FLJ44681; NCAN; NEUR; neurocan proteoglycan; Neurocan

Citations for Recombinant Mouse Neurocan Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Neurocan is a New Substrate for the ADAMTS12 Metalloprotease: Potential Implications in Neuropathies
    Authors: T Fontanil, Y Mohamedi, A Moncada-Pa, T Cobo, JA Vega, JL Cobo, O García-Suá, J Cobo, ÁJ Obaya, S Cal
    Cell. Physiol. Biochem., 2019;52(5):1003-1016.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons
    Authors: CS Sullivan, I Gotthard, EV Wyatt, S Bongu, V Mohan, RJ Weinberg, PF Maness
    Sci Rep, 2018;8(1):6143.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Taxol facilitates axon regeneration in the mature CNS.
    Authors: Sengottuvel V, Leibinger M, Pfreimer M, Andreadaki A, Fischer D
    J. Neurosci., 2011;31(7):2688-99.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse Neurocan Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Neurocan Protein, CF and earn rewards!

Have you used Recombinant Mouse Neurocan Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review