Recombinant Mouse Neurocan Protein, CF Summary
When 5 x 104 cells/well are added to mouse Neurocan coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is
Asp23-Asp637, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 300 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Neurocan is a 220 kDa nervous tissue-specific chondroitin sulfate proteoglycan member of the aggrecan/versican proteoglycan family (1). Mouse Neurocan is synthesized as a 1268 amino acid (aa) precursor that contains a 22 aa signal sequence and a 1246 aa mature chain. The mature chain contains one Ig-like V-type domain (aa 37 - 157), two Link domains (aa 159 - 254 and 258 - 356), two EGF-like domains (aa 960 - 996 and 998 - 1034), one C-type lectin-like domain (aa 1036 - 1165), one Sushi domain (aa 1165 - 1224), and five potential sites for N-linked glycosylation. Mature mouse Neurocan is 90% and 66% aa identical to mature rat and human Neurocan, respectively. Neurocan binds with high affinity to the cell adhesion molecules (CAM) Ng-CAM and N-CAM to inhibit Neuronal adhesion and neurite growth (2 - 3). In the developing rat retina, the expression of Neurocan is regulated both temporally and spatially, which suggests that it may play a role in the differentiation of and neural network formation of the mammalian retina (1). Injury to the CNS leads to permanent loss of function due to the inability of severed nerve fibers to regenerate back to their targets (4). The lack of CNS repair is attributed in part to the extracellular matrix chondroitin sulfate proteoglycans, such as Neurocan, which are produced by activated glial cells post-injury (4).
- Inatani, M. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:2350.
- Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
- Retzler, C. et al. (1996) J. Biol. Chem. 271:27304.
- Quaglia, X. et al. (2008) Brain 131:240.
Citations for Recombinant Mouse Neurocan Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Neurocan is a New Substrate for the ADAMTS12 Metalloprotease: Potential Implications in Neuropathies
Authors: T Fontanil, Y Mohamedi, A Moncada-Pa, T Cobo, JA Vega, JL Cobo, O García-Suá, J Cobo, ÁJ Obaya, S Cal
Cell. Physiol. Biochem., 2019;52(5):1003-1016.
Sample Types: Recombinant Protein
Perineuronal Net Protein Neurocan Inhibits NCAM/EphA3 Repellent Signaling in GABAergic Interneurons
Authors: CS Sullivan, I Gotthard, EV Wyatt, S Bongu, V Mohan, RJ Weinberg, PF Maness
Sci Rep, 2018;8(1):6143.
Sample Types: Whole Cells
Taxol facilitates axon regeneration in the mature CNS.
Authors: Sengottuvel V, Leibinger M, Pfreimer M, Andreadaki A, Fischer D
J. Neurosci., 2011;31(7):2688-99.
Sample Types: Whole Cells
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