Recombinant Mouse NKG2D Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
139-NK-050
Product Details
Citations (18)
FAQs
Supplemental Products
Reviews (1)

Recombinant Mouse NKG2D Fc Chimera Protein, CF Summary

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rmNKG2D/Fc Chimera at 4 µg/mL (100 µL/well) can bind biotinylated rmRae-1 gamma /Fc Chimera with a linear range of 1.56-100 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse NKG2D/CD314 protein
MD Human IgG1
(Pro100 - Lys330)
IEGR Mouse NKG2D
(Phe94 - Val232)
Accession # Q4FJM0
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
42.7 kDa (monomer)
SDS-PAGE
55-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

139-NK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: NKG2D/CD314

NKG2D is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co-stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, δ and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta -2 microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1 - 5).

References
  1. Bauer, S. et al. (1999) Science 285:727.
  2. Wu, J. et al. (1999) Science 285:730.
  3. Diefenbach, A. et al. (2001) Nature 413:165.
  4. Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
  5. NKG2D and its Ligands, www.RnDSystems.com.
Long Name
Natural Killer G2D
Entrez Gene IDs
22914 (Human); 27007 (Mouse)
Alternate Names
CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

Citations for Recombinant Mouse NKG2D Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Impaired downregulation of NKG2D ligands by Nef protein from elite controllers sensitizes HIV-1-infected cells to ADCC
    Authors: N Alsahafi, J Richard, J Prévost, M Coutu, N Brassard, MS Parsons, DE Kaufmann, M Brockman, A Finzi
    J. Virol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. TLR9 Regulates the NF-?B-NLRP3-IL-1? Pathway Negatively in Salmonella-Induced NKG2D-Mediated Intestinal Inflammation
    Authors: Y Li, M Liu, Z Zuo, J Liu, X Yu, Y Guan, R Zhan, Q Han, J Zhang, R Zhou, R Sun, Z Tian, C Zhang
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. T Cells Engineered With Chimeric Antigen Receptors Targeting NKG2D Ligands Display Lethal Toxicity in Mice.
    Authors: VanSeggelen H, Hammill J, Dvorkin-Gheva A, Tantalo D, Kwiecien J, Denisova G, Rabinovich B, Wan Y, Bramson J
    Mol Ther, 2015;23(10):1600-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The MICA-129 dimorphism affects NKG2D signaling and outcome of hematopoietic stem cell transplantation
    EMBO Mol Med, 2015;7(11):1480-502.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. TL1A regulates TCRgammadelta+ intraepithelial lymphocytes and gut microbial composition.
    Authors: Tougaard P, Skov S, Pedersen A, Krych L, Nielsen D, Bahl M, Christensen E, Licht T, Poulsen S, Metzdorff S, Hansen A, Hansen C
    Eur J Immunol, 2015;45(3):865-75.
  6. CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice.
    Authors: Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W
    Immunology, 2014;141(3):401-15.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. The bispecific immunoligand ULBP2-aCEA redirects natural killer cells to tumor cells and reveals potent anti-tumor activity against colon carcinoma.
    Authors: Rothe A, Jachimowicz R, Borchmann S, Madlener M, Kessler J, Reiners K, Sauer M, Hansen H, Ullrich R, Chatterjee S, Borchmann P, Yazaki P, Koslowsky T, Engert A, Heukamp L, Hallek M, von Strandmann E
    Int J Cancer, 2014;134(12):2829-40.
  8. MULT1E/mIL-12: a novel bifunctional protein for natural killer cell activation.
    Authors: Tietje A, Li J, Yu X, Wei Y
    Gene Ther, 2014;21(5):468-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Alloimmune activation promotes anti-cancer cytotoxicity after rat liver transplantation.
    Authors: Lacotte S, Oldani G, Slits F, Orci L, Rubbia-Brandt L, Morel P, Mentha G, Toso C
    PLoS ONE, 2014;9(3):e91515.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair.
    Authors: Yoshida S, Mohamed RH, Kajikawa M, Koizumi J, Tanaka M, Fugo K, Otsuka N, Maenaka K, Yagita H, Chiba H, Kasahara M
    J. Immunol., 2012;188(8):3972-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Expression of WSX1 in tumors sensitizes IL-27 signaling-independent natural killer cell surveillance.
    Authors: Dibra D, Cutrera JJ, Xia X, Birkenbach MP, Li S
    Cancer Res., 2009;69(13):5505-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Essential function for SAP family adaptors in the surveillance of hematopoietic cells by natural killer cells.
    Authors: Dong Z, Cruz-Munoz ME, Zhong MC, Chen R, Latour S, Veillette A
    Nat. Immunol., 2009;10(9):973-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Intrinsic sensor of oncogenic transformation induces a signal for innate immunosurveillance.
    Authors: Unni AM, Bondar T, Medzhitov R
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(5):1686-91.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D.
    Authors: Takada A, Yoshida S, Kajikawa M, Miyatake Y, Tomaru U, Sakai M, Chiba H, Maenaka K, Kohda D, Fugo K, Kasahara M
    J. Immunol., 2008;180(3):1678-85.
    Species: Mouse
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  15. Tumor cells expressing a fusion protein of MULT1 and Fas are rejected in vivo by apoptosis and NK cell activation.
    Authors: Kotturi HS, Li J, Branham-O'Connor M, Stickel SL, Yu X, Wagner TE, Wei Y
    Gene Ther., 2008;15(19):1302-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Generation, affinity maturation, and characterization of a human anti-human NKG2D monoclonal antibody with dual antagonistic and agonistic activity.
    Authors: Kwong KY, Baskar S, Zhang H, Mackall CL, Rader C
    J. Mol. Biol., 2008;384(5):1143-56.
    Species: Mouse
    Sample Types: Antibody
    Applications: Bioassay
  17. Chimeric NKG2D receptor-bearing T cells as immunotherapy for ovarian cancer.
    Authors: Barber A, Zhang T, DeMars LR, Conejo-Garcia J, Roby KF, Sentman CL
    Cancer Res., 2007;67(10):5003-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. DNA-based vaccines activate innate and adaptive antitumor immunity by engaging the NKG2D receptor.
    Authors: Zhou H, Luo Y, Lo JF, Kaplan CD, Mizutani M, Mizutani N, Lee JD, Primus FJ, Becker JC, Xiang R, Reisfeld RA
    Proc. Natl. Acad. Sci. U.S.A., 2005;102(31):10846-51.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Recombinant Mouse NKG2D Fc Chimera Protein, CF
By Anonymous on 09/08/2017
Application: In vivo study