Recombinant Mouse Noggin (aa 28-232) Protein

R&D Systems | Catalog # 6997-NG

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Noggin (aa 28-232) Protein (6997-NG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P97466

Applications

Bioactivity
View all Noggin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Noggin protein
Gln28-Cys232

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln28 predicted, sequencing might be blocked

Predicted Molecular Mass

23 kDa (monomer)

SDS-PAGE

25-35 kDa, reducing conditions

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.04-0.16 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (Catalog # 314-GP).

Reviewed Applications

Read 2 reviews rated 5 using 6997-NG in the following applications:

Scientific Data Images for Recombinant Mouse Noggin (aa 28-232) Protein

Recombinant Mouse Noggin (aa 28‑232) Protein SEC-MALS.

Recombinant Mouse Noggin Protein (Catalog # 6997-NG) has a molecular weight (MW) of 55.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Mouse Noggin (aa 28-232) Protein Bioactivity

Recombinant Mouse Noggin (aa 28-232) Protein Bioactivity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04-0.16 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP-4 (314-BP).

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 6997-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Carrier Free: 6997-NG/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Noggin

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N‑terminal acidic region, a central basic
heparin‑binding segment and a C‑terminal cysteine‑knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of
heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature mouse Noggin shares 99%, 100%, 98%, 97% and 87% aa sequence identity with human, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP‑4 with high affinity and others such as BMP‑7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4). During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine‑knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons (6, 9-13). Noggin also appears to maintain adult stem cell populations in vivo, for example, maintaining neural stem cells within the hippocampus (13).

References

  1. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  2. Groppe, J. et al. (2002) Nature 420:636.
  3. Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089.
  4. Brunet, L. J. et al. (1998) Science 280:1455.
  5. McMahon, J. A. et al. (1998) Genes Dev. 12:1438.
  6. Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24.
  7. Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607.
  8. Gong, Y. et al. (1999) Nat. Genet. 21:302.
  9. Xu, R.-H. et al. (2005) Nat. Methods 2:185.
  10. Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934.
  11. Chaturvedi, G. et al. (2009) Cell Prolif. 42:425.
  12. Chiba, S. et al. (2008) Stem Cells 26:2810.
  13. Bonaguidi, M.A. et al. (2008) J. Neurosci. 28:9194.

Alternate Names

NOG, SYM1, SYNS1, SYNS1A

Entrez Gene IDs

9241 (Human); 18121 (Mouse)

Gene Symbol

NOG

UniProt

P97466

Additional Noggin Products

Product Documents for Recombinant Mouse Noggin (aa 28-232) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 6997-NG (with carrier)
Download SDS 6997-NG/CF (carrier free)

Product Specific Notices for Recombinant Mouse Noggin (aa 28-232) Protein

For research use only

Citations for Recombinant Mouse Noggin (aa 28-232) Protein

Customer Reviews for Recombinant Mouse Noggin (aa 28-232) Protein (2)

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  • Recombinant Mouse Noggin (aa 28-232) Protein
    Name: Anonymous
    Application: Immunostaining
    Verified Customer | Posted 10/26/2018
    Immunostaining with pSmad 1/5/8 after treatment with BMP2 or BMP2 + Noggin
    Recombinant Mouse Noggin (aa 28-232) Protein 6997-NG
  • Recombinant Mouse Noggin (aa 28-232) Protein
    Name: Christine Quach
    Application: 3D Cell Culture
    Verified Customer | Posted 09/08/2017

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