Recombinant Mouse OX40 Ligand/TNFSF4 Protein

Carrier Free

Catalog # Availability Size / Price Qty
1236-OX-025/CF

With Carrier

Catalog # Availability Size / Price Qty
1236-OX-025

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Product Details
Citations (7)
FAQs
Reviews (1)

Recombinant Mouse OX40 Ligand/TNFSF4 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 10-30 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Mouse myeloma cell line, NS0-derived mouse OX40 Ligand/TNFSF4 protein
Gln49-Leu198, with an N-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
18 kDa
SDS-PAGE
21-25 kDa, reducing conditions

Product Datasheets

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1236-OX (with carrier)

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1236-OX/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1236-OX

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1236-OX/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Murine OX40L cDNA encodes a 198 amino acid (aa) residue protein comprised of a 28 aa N-terminal cytoplasmic domain, a 20 aa transmembrane segment, and a 150 aa C-terminal extracellular domain (1). Human and murine OX40L share 46% sequence identity at the amino acid level (1). The OX40L is expressed on activated antigen presenting cells such as B cells, macrophages, dendritic cells, and on endothelial cells at the site of inflammation. The receptor for OX40L is OX40 (CD134) that is expressed predominantly on activated CD4+ T cells. Expression of OX40 is transient following engagement of T cell receptors (2). Ligation of OX40L by OX40 stimulates proliferation and differentiation of activated B cells, and increases immunoglobulin secretion (3, 4). The expression of OX40L on B cells is up-regulated by CD40 ligation (3). Engagement of the OX40-OX40L system has co-stimulatory effects on T cells by stimulating the production of cytokines by T helper cells and increasing the survival of memory T cells (2, 5). Blocking of the OX40-OX40L interaction in vitro inhibits co-stimulation resulting in decreased T cell proliferation and adhesion of T cells to endothelial cells. Inhibition of the OX40-OX40L interaction in disease models has beneficial effects in acute graft-versus-host disease, inflammatory bowel disease and decreases the development of collagen-induced arthritis and experimental leishmaniasis (6).

References
  1. Baum, P.R. et al. (1994) EMBO J. 13:3992.
  2. Gramaglia, I. et al. (1999) J. Immunol. 161:6510.
  3. Stuber, E. et al. (1995) Immunity 2:507.
  4. Malstrom, V. et al. (2001) J. Immunol. 166:6972.
  5. Maxwell, J.R. et al. (2000) J. Immunol. 164:107.
  6. Weinberg, A.D. (2002) Trends Immunol. 23:102.
Entrez Gene IDs
7292 (Human); 22164 (Mouse); 89814 (Rat)
Alternate Names
CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1

Citations for Recombinant Mouse OX40 Ligand/TNFSF4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019-06-11;27(11):3117-3123.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. OX40 Regulates Both Innate and Adaptive Immunity and Promotes Nonalcoholic Steatohepatitis
    Authors: G Sun, H Jin, C Zhang, H Meng, X Zhao, D Wei, X Ou, Q Wang, S Li, T Wang, X Sun, W Shi, D Tian, K Liu, H Xu, Y Tian, X Li, W Guo, J Jia, Z Zhang, D Zhang
    Cell Rep, 2018-12-26;25(13):3786-3799.e4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Nr4a Receptors Regulate Development and Death of Labile Treg Precursors to Prevent Generation of Pathogenic Self-Reactive Cells
    Authors: T Sekiya, S Hibino, K Saeki, M Kanamori, S Takaki, A Yoshimura
    Cell Rep, 2018-08-07;24(6):1627-1638.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Crucial role of OX40/OX40L signaling in a murine model of asthma
    Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
    Mol Med Rep, 2018-01-18;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Guidance of super-enhancers in regulation of IL-9 induction and airway inflammation
    Authors: X Xiao, Y Fan, J Li, X Zhang, X Lou, Y Dou, X Shi, P Lan, Y Xiao, L Minze, XC Li
    J. Exp. Med., 2018-01-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Highly reduced binding to high and low affinity mouse Fc gamma receptors by L234A/L235A and N297A Fc mutations engineered into mouse IgG2a.
    Authors: Arduin E, Arora S, Bamert P, Kuiper T, Popp S, Geisse S, Grau R, Calzascia T, Zenke G, Kovarik J
    Mol Immunol, 2014-10-18;63(2):456-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  7. TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y
    Biochem. Biophys. Res. Commun., 2009-05-04;384(3):296-300.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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Recombinant Mouse OX40 Ligand/TNFSF4 Protein
By Anonymous on 03/24/2018
Application: Binding assay/Protein-protein interaction