Recombinant Mouse OX40/TNFRSF4 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1256-OX-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Mouse OX40/TNFRSF4 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit OX40 Ligand induced IL-2 secretion by mouse T cells. The ED50 for this effect is 0.1-0.3 µg/mL in the presence of 30 ng/mL of Recombinant Mouse OX40 Ligand/TNFSF4 (Catalog # 1236-OX).
Source
Mouse myeloma cell line, NS0-derived mouse OX40/TNFRSF4 protein
Murine OX40
(Val20-Pro211)
Accession # P47741
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48 kDa (monomer)
SDS-PAGE
65-75 kDa, reducing conditions

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1256-OX

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1256-OX

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: OX40/TNFRSF4

OX40 (CD134; TNFRSF4) is a T cell co-stimulatory molecule of the TNF receptor superfamily that coordinates with other co-stimulators (CD28, CD40, CD30, CD27 and 4-1BB) to manage the activation of the immune response (1-3). Mouse OX40 cDNA encodes a 256 amino acid (aa) type I transmembrane glycoprotein with a 19 aa signal sequence, a 192 aa extracellular domain (ECD) that contains a cysteine-rich region, a 25 aa transmembrane segment, and a 36 aa cytoplasmic domain (4). The ECD of mouse OX40 shares 90% and 63% sequence identity with the ECD of rat and human OX40, respectively. OX40 is up-regulated on CD4+ and CD8+ T cells upon engagement of the TCR by antigen presenting cells along with co-stimulation by CD40-CD40 Ligand and CD28-B7 (5, 6). OX40 Ligand is primarily expressed on antigen presenting cells (5). OX40 Ligand engagement of OX40 on activated CD4+ T cells results in increased T cell survival, proliferation, and cytokine production. It also inhibits the conversion of effector T cells into immunosuppressive regulatory T cells (Tregs) and can promote the maintenance of and recall response in memory T cells (3, 7-10). OX40 is constitutively expressed on Tregs and enhances the sensitivity of Tregs to IL-2, thus promoting Treg proliferation. OX40 has also been shown to decrease the cells’ immunosuppressive activity on effector T cells (11-14). OX40-OX40 Ligand signaling is involved in allergic airway inflammation, graft-versus-host disease and autoimmune disease (6, 15, 16). Mutations in OX40 and OX40 Ligand are associated with cardiovascular disease (17, 18).

References
  1. Hori, T. (2006) Int. J. Hematol. 83:17.
  2. Latza, U. et al. (1994) Eur. J. Immunol. 24:677.
  3. Salek-Ardakani, S. et al. (2003) J. Exp. Med. 198:315.
  4. al-Shamkhani, A. et al. (1996) Eur. J. Immunol. 26:1695.
  5. Moran, A.E. et al. (2013) Curr. Opin. Immunol. 25:230.
  6. Gramaglia, I. et al. (1998) J. Immunol. 161:6510.
  7. Xiao, X. et al. (2008) J. Immunol. 181:3193.
  8. So, T. and M. Croft (2007) J. Immunol. 179:1427.
  9. Mousavi, S.F. et al. (2008) J. Immunol. 181:5990.
  10. Bansal-Pakala, P. et al. (2001) Nat. Med. 7:907.
  11. Piconese, S. et al. (2010) Eur. J. Immunol. 40:2902.
  12. Griseri, T. et al. (2010) J. Exp. Med. 207:699.
  13. Xiao, X. et al. (2012) J. Immunol. 188:892.
  14. Vu, M.D. et al. (2007) Blood 110:2501.
  15. Damayanti, T. et al. (2010) Am. J. Respir. Crit. Care Med. 181:688.
  16. Xiao, X. et al. (2012) Nat. Immunol. 13:981.
  17. Nakano, M. et al. (2010) Cardiovasc. Res. 88:539.
  18. Ishii, N. et al. (2010) Adv. Immunol. 105:63.
Entrez Gene IDs
7293 (Human); 22163 (Mouse); 25572 (Rat); 102145978 (Cynomolgus Monkey)
Alternate Names
ACT-135; ACT35 antigen; ACT35ATC35 antigen; CD134 antigen; CD134; Ly-70; OX40 cell surface antigen; OX40 homologue; OX40; OX40L receptor; OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; TNFRSF4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen

Citations for Recombinant Mouse OX40/TNFRSF4 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019;27(11):3117-3123.e5.
    Species: Mouse
    Sample Types: In Vivo
    Applications: mAb Generation
  2. OX40 Regulates Both Innate and Adaptive Immunity and Promotes Nonalcoholic Steatohepatitis
    Authors: G Sun, H Jin, C Zhang, H Meng, X Zhao, D Wei, X Ou, Q Wang, S Li, T Wang, X Sun, W Shi, D Tian, K Liu, H Xu, Y Tian, X Li, W Guo, J Jia, Z Zhang, D Zhang
    Cell Rep, 2018;25(13):3786-3799.e4.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Assembling OX40 aptamers on a molecular scaffold to create a receptor-activating aptamer.
    Authors: Dollins CM, Nair S, Boczkowski D, Lee J, Layzer JM, Gilboa E, Sullenger BA
    Chem. Biol., 2008;15(7):675-82.
    Species: Mouse
    Sample Types: RNA
    Applications: Bioassay

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Reviews for Recombinant Mouse OX40/TNFRSF4 Fc Chimera Protein, CF

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Recombinant Mouse OX40/TNFRSF4 Fc Chimera Protein, CF
By Anonymous on 06/25/2019
Application: Binding assay/Protein-protein interaction
Reason for Rating: Analysis of the kinetics of the interaction between CD3-OX40Land OX40

Recombinant Mouse OX40/TNFRSF4 Fc Chimera Protein, CF
By Anonymous on 01/31/2019
Application: Binding assay/Protein-protein interaction