Recombinant Mouse PDGF R beta His-tag Protein, CF

Catalog # Availability Size / Price Qty
10066-PR-050
Recombinant Mouse PDGF R beta His-tag Protein, CF Binding Activity
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Recombinant Mouse PDGF R beta His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat PDFG-BB (Catalog # 520-BB/CF) is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse PDGF R beta that produces 50% of the optimal binding response is 0.25-1.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse PDGF R beta protein
Leu32-Lys530, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu32
Predicted Molecular Mass
57 kDa
SDS-PAGE
89-103 kDa, reducing conditions

Product Datasheets

Data Images

Binding Activity Recombinant Mouse PDGF R beta His-tag Protein, CF Binding Activity View Larger

When Recombinant Rat PDGF‑BB Homodimer (Catalog # 520-BB/CF) is coated at 2 μg/mL (100 μL/well), Recombinant Mouse PDGF R beta (Catalog # 10066-PR) binds with an ED50 of 0.25-1.5 μg/mL.

SDS-PAGE Recombinant Mouse PDGF R beta His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse PDGF R beta was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 89-103 kDa.

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Background: PDGF R beta

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta  receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF R alpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG-63 (5). Both the recombinant mouse and human soluble PDGF R alpha bind PDGF with high affinity and are potent PDGF antagonists.

References
  1. Betshotz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
  5. Tiesman, J. and C.E. Hart (1993) J. Biol. Chem. 5:9621.
Long Name
Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs
5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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