Recombinant Mouse Plexin A1 Protein, CF

Catalog # Availability Size / Price Qty
4309-PA-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Plexin A1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When recombinant mouse Plexin A1 is coated at 2.5 μg/mL (100 μL/well), recombinant human Semaphorin 6C/Fc Chimera binds with an apparent KD <5 nM.
Source
Mouse myeloma cell line, NS0-derived mouse Plexin A1 protein
Ser28-Pro1242, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser28
Predicted Molecular Mass
134.5 kDa
SDS-PAGE
150-160 kDa, reducing conditions

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4309-PA

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4309-PA

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Plexin A1

Plexin A1 (formerly Plexin 1) is a 200 kDa type I transmembrane protein that is a member of the Plexin family of Semaphorin signal transducers (1). Plexin signaling induces cytoskeletal remodeling, which mediates cell migration and axon repulsion (2). The mouse Plexin A1 cDNA encodes 1894 amino acids (aa) including a 27 aa signal sequence, a 1215 aa extracellular domain (ECD) with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain (1). Within the ECD, human Plexin A1 shares 95%, 95%, 92%, 80% and 79% aa sequence identity with mouse, rat, bovine, chicken and Xenopus Plexin A1, respectively. The four mouse Plexin A molecules share 59 ‑ 67% aa identity with each other. Plexin A1 binds Class 3 (secreted) Semaphorins indirectly via Neuropilin (Npn)-1 and Npn-2, and binds transmembrane Semaphorin 6D directly (3 ‑ 5). Sema3A engagement of Plexin A1 and Npn-1 guides proprioceptive and sensory neurons during development, while Sema3B engagement guides floorplate neurons (5 ‑ 8). In contrast, T cell Sema6D engagement of dendritic cell Plexin A1 controls actin polymeration, which supports formation of immunological synapses and enhances the function of the dendritic cells (3, 4, 9). Complex formation with DAP12 allows Plexin A1 signaling through TREM family proteins (10, 11). However, the most striking effect of Plexin A1 deletion is on bone homeostasis, where Plexin A1-deficient mice show increased trabecular bone mass due to downregulated osteoclast differentiation (10). Plexin A1 and Sema6D are frequently expressed in malignant pleural mesothelioma, where they promote anchorage-independent growth through complexing with and activating VEGF R2 (12).

References
  1. Kameyama, T. et al. (1996) Biochem. Biophys. Res. Commun. 226:524.
  2. Kruger, R.P. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:789.
  3. Takamatsu, H. et al. (2010) Cell. Mol. Immunol. 7:83.
  4. O’Connor, B.P. and J.P.Y. Ting (2008) Immunol. Res. 41:217.
  5. Takahashi, T. et al. (1999) Cell 99:59.
  6. Yoshida, Y. et al. (2006) Neuron 52:775.
  7. Toyofuku, T. et al. (2005) Nat. Neurosci. 8:1712.
  8. Nawabi, H. et al. (2010) Genes Dev. 24:396.
  9. Eun, S-Y. et al. (2006) J. Immunol. 177:4271.
  10. Takegahara, N. et al. (2006) Nat. Cell Biol. 8:615.
  11. Watarai, H. et al. (2008) Proc. Natl. Acad. Sci. USA 105:2993.
  12. Catalano, A. et al. (2009) Cancer Res. 69:1485.
Entrez Gene IDs
5361 (Human); 18844 (Mouse)
Alternate Names
NOV; NOVP; Plexin A1; PLEXIN-A1; PLXN1; PLXNA1

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