Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Carrier Free

Catalog # Availability Size / Price Qty
7150-RS-025/CF
7150-RS-050/CF
7150-RS-250/CF
7150-RS-010/CF

With Carrier

Catalog # Availability Size / Price Qty
7150-RS-025
7150-RS-050
7150-RS-250
Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein Bioactivity
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (3)

Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 1 protein
Met1-Gln265
Accession #
N-terminal Sequence
Analysis
Ser21
Predicted Molecular Mass
27 kDa
SDS-PAGE
40-43 kDa, reducing conditions

Product Datasheets

7150-RS (with carrier)

7150-RS/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7150-RS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7150-RS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein Bioactivity View Larger

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (1324-WN).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members (1, 2). All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns (1-3). R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization (4). However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly (4-6). R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 7). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (7, 8). Mutations in R-Spondin 1 are also linked with palmoplantar keratoderma, abnormal thickening of the skin on the palms of the hands and soles of the feet (7, 8). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (9). Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (9, 10). Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids (11).

Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization (2, 12). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (13). Mouse R‑Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively, within aa 21‑209.

References
  1. Chen, J.-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  5. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  6. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  7. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
  8. Parma, P. et al. (2006) Nat. Genet. 38:1304.
  9. Kim, K.-A. et al. (2005) Science 309:1256.
  10. Zhao, J. et al. (2007) Gastroenterology 132:1331.
  11. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
  12. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  13. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
Long Name
Roof Plate-specific Spondin 1
Entrez Gene IDs
284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)
Alternate Names
Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1

Citations for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1
    Authors: K Dunbar, A Valanciute, A Lima, PF Vinuela, T Jamieson, V Rajasekara, J Blackmur, AM Ochocka-Fo, A Guazzelli, P Cammareri, MJ Arends, OJ Sansom, KB Myant, SM Farrington, MG Dunlop, FVN Din
    Cell Mol Gastroenterol Hepatol, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Cell Culture
  2. Gut microbiota maturation during early human life induces enterocyte proliferation via microbial metabolites
    Authors: MW Dougherty, O Kudin, M Mühlbauer, J Neu, RZ Gharaibeh, C Jobin
    BMC Microbiol., 2020;20(1):205.
    Species: Mouse
    Sample Types: Organoid
    Applications: Bioassay
  3. Indispensable role of the Ubiquitin-fold modifier 1-specific E3 ligase in maintaining intestinal homeostasis and controlling gut inflammation
    Authors: Y Cai, G Zhu, S Liu, Z Pan, M Quintero, CJ Poole, C Lu, H Zhu, B Islam, JV Riggelen, D Browning, K Liu, R Blumberg, N Singh, H Li
    Cell Discov, 2019;5(0):7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. PDGFR? + pericryptal stromal cells are the critical source of Wnts and RSPO3 for murine intestinal stem cells in vivo
    Authors: G Greicius, Z Kabiri, K Sigmundsso, C Liang, R Bunte, MK Singh, DM Virshup
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Essential role of IFN-? in T cell-associated intestinal inflammation
    Authors: Y Eriguchi, K Nakamura, Y Yokoi, R Sugimoto, S Takahashi, D Hashimoto, T Teshima, T Ayabe, ME Selsted, AJ Ouellette
    JCI Insight, 2018;3(18):.
    Species: Mouse
    Sample Types: Intestinal organoids
    Applications: Bioassay
  6. RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
    Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
    Oncotarget, 2016;7(39):63767-63778.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Trop2 marks transient gastric fetal epithelium and adult regenerating cells after epithelial damage
    Authors: Marie-Isabelle Garcia
    Development, 2016;143(9):1452-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein

Average Rating: 4.7 (Based on 3 Reviews)

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Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein
By Anonymous on 06/16/2020
Application: CellProlif

Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein
By Anonymous on 12/22/2017
Application: CellProlif

Recombinant Mouse R-Spondin 1 (CHO-expressed) Protein
By Anonymous on 05/18/2016
Application: Cell migration/motility