Recombinant Mouse R-Spondin 2 Protein

Carrier Free

Catalog # Availability Size / Price Qty
6946-RS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
6946-RS-025
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Citations (5)
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Recombinant Mouse R-Spondin 2 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.4-2.4 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Chinese Hamster Ovary cell line, CHO-derived mouse R-Spondin 2 protein
Met1-Gly205
Accession #
N-terminal Sequence
Analysis
Predicted Gln22, not observed that sequencing might be blocked & Ala32
Predicted Molecular Mass
21 kDa & 20 kDa
SDS-PAGE
26-27 kDa, reducing conditions

Product Datasheets

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6946-RS (with carrier)

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6946-RS/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6946-RS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6946-RS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: R-Spondin 2

Roof plate-specific Spondin 2 isoform 1 (R‑Spondin 2, RSPO2), also known as cysteine‑rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R‑Spondin family (1‑3). The four R‑Spondins regulate Wnt/ beta -catenin signaling and overlap in expression and function (1‑3). Like other R‑Spondins, RSPO2 contains two adjacent cysteine‑rich furin-like domains (aa 90‑134) followed by a thrombospondin (TSP-1) motif (aa 144‑204) and a C-terminal region rich in basic residues (aa 207‑243). The basic region binds heparin and mediates cell surface retention and extracellular matrix attachment while the furin-like domains are required for Wnt/ beta -catenin signaling (1, 3, 4). RSPO2 contains one potential N‑glycosylation site. Mature mouse RSPO2 shares 97‑98% aa identity with human, rat, equine, canine and bovine RSPO2 and ~40% aa identity with RSPO1, RSPO3 and RSPO4. One potential 237 aa mouse isoform diverges after aa 206 and lacks a basic region (5). Human RSPO2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is down‑regulated in tumors of these tissues (1). In the embryonic mouse, RSPO2 expression is concentrated in the apical epidermal ridge, hippocampus, and developing muscle, teeth and bones (1, 6). Deletion of RSPO2 results in down‑regulation of Wnt activity in these areas, malformations of the facial skeleton and limbs, and respiratory failure at birth (7-9). RSPO2 is an extracellular potentiator of Wnt/ beta -catenin signaling (3, 4). It functions at least in part by binding LRP-6, stimulating its long-term phosphorylation and down‑regulating its internalization (3, 4). RSPO proteins, especially RSPO2 and RSPO3, also antagonize DKK1 activity by interfering with DKK1‑mediated LRP-6 and Kremen association (10).

References
  1. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  4. Li, S-J. et al. (2009) Cell Signal. 21:916.
  5. Entrez accession # EDL08755.
  6. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  7. Yamada, W. et al. (2009) Biochem. Biophys. Res. Commun. 381:453.
  8. Jin, Y.-R. et al. (2011) Dev. Biol. 352:1.
  9. Nam, J.-S. et al. (2007) Dev. Biol. 311:124.
  10. Kim, K.-A. et al. (2007) Mol. Biol. Cell 19:2588.
Long Name
Roof Plate-specific Spondin 2
Entrez Gene IDs
340419 (Human); 239405 (Mouse)
Alternate Names
Cristin 2; CRISTIN2; hRspo2; MGC35555; MGC43342; Roof plate-specific spondin-2; RSPO2; R-spondin 2 homolog (Xenopus laevis); RSpondin 2; R-Spondin 2; R-spondin-2

Citations for Recombinant Mouse R-Spondin 2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Neuropilin 1 regulates bone marrow vascular regeneration and hematopoietic reconstitution
    Authors: CM Termini, A Pang, T Fang, M Roos, VY Chang, Y Zhang, NJ Setiawan, L Signaevska, M Li, MM Kim, O Tabibi, PK Lin, JP Sasine, A Chatterjee, R Murali, HA Himburg, JP Chute
    Nature Communications, 2021;12(1):6990.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs
    Authors: M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie
    PLoS ONE, 2020;15(3):e0229445.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC Control
  3. Ectonucleotide pyrophosphatase 2 (ENPP2) plays a crucial role in myogenic differentiation through the regulation by WNT/beta-Catenin signaling
    Authors: JP Sah, NTT Hao, X Han, TTT Tran, S McCarthy, Y Oh, JK Yoon
    Int. J. Biochem. Cell Biol., 2019;118(0):105661.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Identification of genes which regulate stroma-dependent in vitro hematopoiesis
    Authors: P Periasamy, V Tran, HC O'Neill
    PLoS ONE, 2018;13(10):e0205583.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
    Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
    Oncotarget, 2016;7(39):63767-63778.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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