Recombinant Mouse RGM-A Protein, CF

Catalog # Availability Size / Price Qty
2458-RG-050
Product Details
Citations (5)
FAQs
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Recombinant Mouse RGM-A Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human BMP-4 (Catalog # 314-BP) is coated at 1 μg/mL (100 μL/well), Recombinant Mouse RGM-A binds with an apparent Kd <50 nM.
Source
Mouse myeloma cell line, NS0-derived mouse RGM-A protein
Mouse RGM-A
Cys48-Gly421 &
Pro170-Gly421
Accession # Q6PCX7
DI 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Cys48 (proprotein) & Pro170 (mature)
Predicted Molecular Mass
42.5 kDa
SDS-PAGE
Multiple bands between 25-37 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2458-RG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: RGM-A

Mouse repulsive guidance molecule (RGM‑A) is a 33 kDa GPI-linked member of an expanding RGM‑related family of neuronal and muscle-expressed membrane proteins (1, 2). It is synthesized as a 454 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 122 aa N-terminal prosegment, a 258 mature region (aa 170-427) and a 27 aa C-terminal prosegment (3, 4). The N-terminal prosegment contains an RGD tripeptide and the molecule's only two potential N‑linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid‑proline bond, creating a predicted 32 kDa mature region (3). There is one potential alternative start site at Met17. The mature region of mouse RGM-A is 93% and 87% aa identical to human and chick mature region RGM-A, respectively. Recognizing that RGM-A possesses a cleavage site between Asp169Pro170, it is suggested that membrane-expressed RGM-A may exist as a covalent heterodimer that links a GPI-anchored mature molecule with its prosegment (1, 4, 5). When compared to mouse RGM-B and C, the mature region of mouse RGM-A shows 77% and 76% aa identity, respectively (5, 6). Cells normally described as expressing RGM-A include neurons and oligodendroglia. But this select group is expanded to endothelium, microglia and leukocytes in areas of neuronal injury (7). Functionally, recombinant chick RGM has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mice, RGM-A also induces growth cone collapse on primary DRG neurons (8). And in a mouse culture system, chick RGM-A is reported to be responsible for the layered segmentation of entorhinal cortical projections to the hippocampus (9). RGM reportedly binds to neogenin and BMP-2/4, with the latter binding occuring within the context of a BMPRI:ActRIIA:RGM-A receptor complex (10-13).

References
  1. Severyn, C.J. et al. (2009) Biochem. J. 422:393.
  2. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  3. Schmidtmer, J. et al. (2004) Gene Expr. Patterns 4:105.
  4. Matsunaga, E. & A. Chedotal (2004) Develop. Growth Differ. 46:481.
  5. Monnier, P.P. et al. (2002) Nature 419:392.
  6. Niederkofler V. et al. (2004) J. Neurosci. 24:808.
  7. Hata, K. et al. (2006) J. Cell Biol. 173:47.
  8. Conrad, S. et al. (2007) J. Biol. Chem. 282:16423.
  9. Brinks, H. et al. (2004) J. Neurosci. 24:3862.
  10. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
  11. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
  12. Babitt, J.L. et al. (2005) J. Biol. Chem. 280:29820.
  13. Xia, Y. et al. (2007) J. Biol. Chem. 282:18129.
Long Name
Repulsive Guidance Molecule A
Entrez Gene IDs
56963 (Human); 244058 (Mouse)
Alternate Names
RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A

Citations for Recombinant Mouse RGM-A Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. RGMb is a novel binding partner for PD-L2 and its engagement with PD-L2 promotes respiratory tolerance.
    Authors: Xiao, Yanping, Yu, Sanhong, Zhu, Baogong, Bedoret, Denis, Bu, Xia, Francisco, Loise M, Hua, Ping, Duke-Cohan, Jonathan, Umetsu, Dale T, Sharpe, Arlene H, DeKruyff, Rosemari, Freeman, Gordon J
    J Exp Med, 2014;211(5):943-59.
    Species: Mouse
    Sample Types: Antibody
    Applications: ELISA (Capture)
  2. RGMa regulates cortical interneuron migration and differentiation.
    Authors: O'Leary, Conor, Cole, Stacey J, Langford, Michael, Hewage, Jayani, White, Amanda, Cooper, Helen M
    PLoS ONE, 2013;8(11):e81711.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Repulsive guidance molecule b inhibits neurite growth and is increased after spinal cord injury.
    Authors: Liu X, Hashimoto M, Horii H, Yamaguchi A, Naito K, Yamashita T
    Biochem. Biophys. Res. Commun., 2009;382(4):795-800.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Peptides derived from repulsive guidance molecule act as antagonists.
    Authors: Suda M, Hata K, Sawada A, Nakamura Y, Kubo T, Yamaguchi A, Yamashita T
    Biochem. Biophys. Res. Commun., 2008;371(3):501-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. LIM-only protein 4 interacts directly with the repulsive guidance molecule A receptor Neogenin.
    Authors: Schaffar G, Taniguchi J, Brodbeck T, Meyer AH, Schmidt M, Yamashita T, Mueller BK
    J. Neurochem., 2008;107(2):418-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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