Recombinant Mouse RP105/CD180 Fc Chimera Protein, CF

Recombinant Mouse RP105/CD180 Fc Chimera Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: RP105/CD180

Radioprotective, 105 kDa (RP105), also known as CD180 and lymphocyte antigen 64 (LY64), is a type I transmembrane glycoprotein with extracellular leucine-rich repeats (LRR) typically found in Toll-like receptor (TLR) family members. However, it has a short cytoplasmic tail and lacks the Toll-IL-1R (TIR) domain that defines the IL-1 R/TLR superfamily. Mouse RP105 cDNA encodes a 661 amino acid (aa) residues  protein with a 20 aa signal peptide, a 606 aa extracellular domain, a 24 aa transmembrane domain and an 11 aa cytoplasmic region (1). It shares 78% aa sequence identity with its human counterpart (2). RP105 is expressed primarily on mature B cells and macrophages. The extracellular domain of RP105 associates with a secreted protein MD-1 (also known as LY 86) that is required for efficient RP105 cell surface expression and function (3). Cell surface RP105/MD-1 complex, in conjunction with TLR4, mediates the innate immune response to bacterial lipopolysaccharide (LPS) in B cells. Activation of the RP105 complex has been shown to protect against apoptosis, induce B-cell proliferation, and up-regulate B7.2, a co-stimulatory molecule (4, 5).

Related Research Areas

• B Cell Activation
• Dendritic Cell Pathogen Recognition/Uptake
• Immune Regulatory Molecules on Endothelial Cells
• Monocyte Markers
• Toll-Like Receptors