Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF Summary
Accession # P27005
Accession # P31725
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and DTT.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse S100A8/S100A9 (Catalog #
8916-S8) induces IL-6 secretion by A375 human melanoma cells. The ED50 for this effect is
1 μg/lane of Recombinant Mouse S100A8/S100A9 was resolved with
SDS-PAGE under reducing (R) and
non-reducing (NR) conditions and visualized by silver staining, showing R bands at
6.3, 10.8 kDa and NR band at 6, 9.6 kDa.
Background: S100A8/S100A9 Heterodimer
S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). The 10 kDa S100A8 and 14 kDa S100A9 each contain two EF-hand calcium binding motifs. Mouse S100A8 shares 57% and 80% amino acid (aa) sequence identity with human and rat S100A8, respectively. Mouse S100A9 shares 57% and 79% amino acid sequence identity with human and rat S100A9, respectively (3, 4). S100A8 and S100A9 can associate into non-covalent homodimers and 34-35 kDa heterodimers known as S100A8/9 or Calprotectin. The presence of calcium and zinc also induces the formation of the tetramer (5, 6). S100A8 and S100A9 are up-regulated in neutrophils, monocytes, Schwann cells, and keratinocytes at sites of inflammation (7-13). The S100A8/9 heterodimer is elevated in rheumatoid arthritis synovial fluid and in the serum of cardiovascular disease, atopic dermatitis, and psoriatic arthritis patients (1, 9, 13-15). It exerts its effects through the receptors RAGE and TLR4 (11, 16). The heterodimer promotes neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (5, 8, 9, 12). It promotes astrocyte proliferation (16) and the suppression of tumor growth by promoting the influx and activation of NK cells (10, 11). The S100A8/9 heterodimer additionally binds to unsaturated fatty acids such as arachidonic acid (7), and it sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (17).
- Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
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- Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
- Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
- Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
- Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
- Zhang, L. et al. (2015) Mol. Med. Rep. 11:4093.
- Narumi, K. et al. (2015) J. Immunol. 194:5539.
- Chernov, A.V. et al. (2015) J. Biol. Chem. 290:11771.
- Jin, S. et al. (2014) Exp. Dermatol. 23:938.
- Vogl, T. et al. (2014) Nat. Commun. 5:4593.
- Hansson, C. et al. (2014) J. Immunol. Res. 2014:696415.
- Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948.
- Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841.
Citations for Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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S100A8/A9 induces microglia activation and promotes the apoptosis of oligodendrocyte precursor cells by activating the NF-?B signaling pathway
Authors: M Wu, L Xu, Y Wang, N Zhou, F Zhen, Y Zhang, X Qu, H Fan, S Liu, Y Chen, R Yao
Brain Res. Bull., 2018;0(0):.
Sample Types: Whole Cells
A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing
Authors: A Basu, S Munir, MA Mulaw, K Singh, B Herold, D Crisan, A Sindrilaru, N Treiber, M Wlaschek, M Huber-Lang, F Gebhard, K Scharffett
Sci Rep, 2018;8(1):6205.
Sample Types: Whole Cells
Hippocampal Mrp8/14 signaling plays a critical role in the manifestation of depressive-like behaviors in mice
Authors: H Gong, WJ Su, ZY Cao, YJ Lian, W Peng, YZ Liu, Y Zhang, LL Liu, R Wu, B Wang, T Zhang, YX Wang, CL Jiang
J Neuroinflammation, 2018;15(1):252.
Sample Types: In Vivo
Applications: In Vivo
Advanced glycation end products impair NLRP3 inflammasome-mediated innate immune responses in macrophages
Authors: S Son, I Hwang, SH Han, JS Shin, OS Shin, JW Yu
J. Biol. Chem., 2017;0(0):.
Sample Types: Whole Cells
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