Recombinant Mouse Semaphorin 6A Fc Chimera Protein, CF Summary
|Mouse Sema 6A|
Accession # O35464
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in MES and NaCl with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Semaphorin 6A
Semaphorin 6A (Sema6A) is an approximately 120 kDa member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, dendritic spine formation, and angiogenesis (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain a characteristic extracellular beta propeller N-terminal semaphorin (sema) domain and also an extracellular plexin-semaphorin-integrin (PSI) domain (1, 2). Within the ECD, mouse Sema6A shares 94% and 98% aa sequence identity with human and rat Sema6A, respectively. Alternative splicing generates additional isoforms with a 26 aa deletion within the sema domain or a 55 aa deletion between the sema domain and transmembrane segment. Sema6A interacts with Plexin A4 (3-7) to induce growth cone collapse and regulate the axon pathfinding of sympathetic neurons (3, 7, 8), cerebellar cortex granule cells (9), hippocampus CA3 region mossy fibers (6), corticospinal tract axons (5, 10), and retinal neurons (4). Plexin A2 can compete with Plexin A4 for Sema6A binding and thereby limit axon repulsion (6). In addition, Sema6A and Plexin A4 are co-expressed on dorsal root ganglion sensory neurons and associate in cis; this prevents in trans interactions and subsequent axon repulsion (7). Sema6A is additionally expressed on vascular endothelial cells where it regulates VEGF R2 responsiveness during angiogenesis (11). In contrast, exogenous Sema6A can inhibit the survival of the HUVEC cell line in a Plexin A4 independent manner (11, 12). Sema6A is also expressed on myelinating oligodendrocytes, Langerhans cells, and some dendritic cells (13, 14).
- Jongbloets, B.C. and R.J. Pasterkamp (2014) Development 141:3292.
- Zhou, L. et al. (1997) Mol. Cell. Neurosci. 9:26.
- Suto, F. et al. (2005) J. Neurosci. 25:3628.
- Matsuoka, R.L. et al. (2011) Nature 470:259.
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- Haklai-Topper, L. et al. (2010) EMBO J. 29:2635.
- Xu, X.M. et al. (2000) J. Neurosci. 20:2638.
- Kerjan, G. et al. (2005) Nat. Neurosci. 8:1516.
- Mauti, O. et al. (2007) Neural Dev. 2:28.
- Segarra, M. et al. (2012) Blood 120:4104.
- Kigel, B. et al. (2011) Blood 118:4285.
- Bernard, F. et al. (2012) Glia 60:1590.
- Gautier, G. et al. (2006) Am. J. Pathol. 168:453.
Citation for Recombinant Mouse Semaphorin 6A Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The Spinal Transcriptome after Cortical Stroke: In Search of Molecular Factors Regulating Spontaneous Recovery in the Spinal Cord
Authors: J Kaiser, M Maibach, I Salpeter, N Hagenbuch, VBC de Souza, MD Robinson, ME Schwab
J. Neurosci., 2019;39(24):4714-4726.
Sample Types: Whole Cells
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