Recombinant Mouse Serglycin/SRGN His-tag Protein, CF

Catalog # Availability Size / Price Qty
10190-SN-050
Recombinant Mouse Serglycin/SRGN His-tag Protein, CF Binding Activity
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Recombinant Mouse Serglycin/SRGN His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD44 Fc Chimera (Catalog # 6127-CD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Mouse Serglycin/SRGN His-tag (Catalog # 10190-SN) binds with an ED50 of 1.5-15 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse Serglycin/SRGN protein
Mouse Serglycin/SRGN
(Tyr26-Ile152)
Accession # P13609
HPGGGSGGGSGGGSHHHHHH
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Tyr26
Predicted Molecular Mass
16 kDa
SDS-PAGE
55 kDa and above, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10190-SN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Mouse CD44 Fc Chimera (Catalog # 6127-CD) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Mouse Serglycin/SRGN His-tag (Catalog # 10190-SN) binds withan ED50 of 1.5‑15 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Serglycin/SRGN His-tag (Catalog # 10190-SN) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining. Due to heavily O-linked glycosylation, it runs as a smear above 55 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serglycin/SRGN

Serglycin, also known as hematopoetic proteoglycan core protein or secretory granule proteoglycan core protein, is a 16 kDa polypeptide with extensive glycosylation modification. Proteoglycans (PGs) are synthesized by all cells and distributed in all tissues. There are three main groups of PGs based on localization: the cell-surface associated PGs, such as Syndecans and Glypicans, the matrix secreted PGs, such as Versican and Perlecan, and the intracellular PGs, with Serglycin being the only known member of this subfamily to date (1). Serglycin was initially found as a hematopoietic PG present in intracellular secretory compartments. Recent studies suggest that Serglycin is expressed by a variety of cell types, where it participates in both physiological functions and pathologic conditions (2, 3). Serglycin is important in formation of secretory granules and mediates storage of various compounds in secretory vesicles. In connective tissue and mucosal mast cells, Serglycin is required for storage and secretion of chemicals and proteases such as histamine, chymase, tryptase and carboxypeptidase (4). Serglycin is also important for storage of granzyme B in T-lymphocytes and localizing neutrophil elastase in azurophil granules of neutrophils (5). It has been found that Serglycin regulates the secretion of TNF-alpha in macrophages (6). Serglycin consists of a small core protein containing serine/glycine repeats, with eight such G-S repeats found in human Serglycin and ten in mouse Serglycin (1). Each serine of this repeat region is a potential GAG attachment site. The size of the PG varies according to the GAG chain length, number, and type (7). In connective tissue mast cells the covalently attached glycosaminoglycan is heparin, whereas mucosal mast cells and activated macrophages contain oversulfated chondroitin sulfate (type E) (8). Within the core protein, mouse Serglycin shares 66% and 38% amino acid identity to rat and human Serglycin. Serglycin can inhibit both the classical and lectin pathways of complement through direct interaction with C1q and mannose-binding lectin (9). In vitro study using human umbilical vein endothelial cells (HUVECs) suggested lipopolysaccharide and interleukin-1 beta played important role in Serglycin synthesis and secretion (10). In addition, Serglycin can promote myeloma cell adhesion to bone marrow stromal cells as well as of non-small cell lung cancer cell migration, invasion and its colonization in the lung and liver through CD44 (11, 12). Serglycin is directly involved in myeloma tumor progression thus suggesting it may be a therapeutic target for multiple myeloma (11).

References
  1. Theocharis, A.D. et al. (2010) FEBS J. 277:3904.
  2. Kolset, S.O. and Pejler G. J. (2011) Immunol. 187:4927.
  3. Scully, O.J. et al. (2012) Anat Rec (Hoboken) 295: 1415.
  4. Abrink, M. et al. (2004) J. Biol. Chem. 279:40897.
  5. Grujic, M. et al. (2005) J. Biol. Chem. 280:33411.
  6. Zernichow, L. et al. (2006) J. Biol. Chem. 281:26792.
  7. Schick, B.P. (2010) Prog Mol Biol Transl Sci. 93:235.
  8. Toyama-Sorimachi, N. et al. (1997) J Biol Chem. 272:26714.
  9. Skliris, A. et al. (2011) Eur. J. Immunol. 41:437.
  10. Reine, T. et al. (2014) Biochim Biophys Acta. 1840:2498.
  11. Purushothaman, A. (2014) J. Biol. Chem. 289:5499.
  12. Guo, J. et al. (2017) Oncogene 36:2457.
Long Name
Hematopoietic Proteoglycan Core Protein
Entrez Gene IDs
5552 (Human); 19073 (Mouse)
Alternate Names
FLJ12930; Hematopoetic proteoglycan core protein; MGC9289; P.PG; Platelet proteoglycan core protein; platelet proteoglycan protein core; PPG; PRG1p.PG; PRGhematopoetic proteoglycan core peptide; proteoglycan 1, secretory granule; proteoglycan protein core for mast cell secretory granule; secretory granule proteoglycan 1; secretory granule proteoglycan core peptide; Secretory granule proteoglycan core protein; serglycin proteoglycan; Serglycin; SRGN

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