Recombinant Mouse SIRP beta 1a His-tag Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Mouse SIRP beta 1a His-tag Protein Binding Activity
2 Images
Product Details

Recombinant Mouse SIRP beta 1a His-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP beta 1a His-tag (Catalog # 10289-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP‑D (Catalog # 1920-SP) binds with an ED50 of 30-180 ng/mL.
Mouse myeloma cell line, NS0-derived mouse SIRP beta 1a protein
Val28-Gly360, with a C-teminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
38 kDa
48-62 kDa, under reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse SIRP beta 1a His-tag Protein Binding Activity View Larger

When Recombinant Mouse SIRP beta 1a His-tag (Catalog # 10289-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP-D (Catalog # 1920-SP) binds with an ED50 of 30-180 ng/mL.

SDS-PAGE Recombinant Mouse SIRP beta 1a His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse SIRP beta 1a His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-62 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: SIRP beta 1a

Signal-regulatory protein beta 1a (SIRP beta 1a) is a disulfide-linked type I membrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. Positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3). Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages (4). Mouse SIRP beta 1a shares 57% and 59% amino acid sequence identity with human SIRP beta 1 and rat SIRP alpha, respectively. SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induce tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5, 6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma receptor-dependent or -independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).

  1. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. van den Berg, T. et al. (2008) Trends in Immunology 29:203.
  3. Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132.
  4. Matozaki, T. et al. (2009) Trends in Cell Biology 19:72.
  5. Dietrich, J. et al. (2000) J Immunol. 164:9.
  6. Brook, G. et al. (2004) J. Immunol. 173:2562.
  7. Hayashi, A. et al. (2004) J Biol Chem. 279:29450.
  8. Fournier, B. et al. (2012) J. Biol. Chem. 287:19386.
  9. Seiffert, M. et al. (2001) Blood 97:2741.
Long Name
Signal-regulatory Protein beta 1A
Entrez Gene IDs
320832 (Mouse)
Alternate Names
SIRP beta 1a; SIRPB1A


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse SIRP beta 1a His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse SIRP beta 1a His-tag Protein, CF and earn rewards!

Have you used Recombinant Mouse SIRP beta 1a His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review