Recombinant Mouse SLAM/CD150 Protein, CF

Name: Recombinant Mouse SLAM/CD150 Protein, CF
Brand: R&D Systems
SKU: 4330-SL
Price: 454 USD
Availability: InStock
Rating: 4 (1 reviews)

R&D Systems | Catalog # 4330-SL

(1)

Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems NS0-derived Recombinant Mouse SLAM/CD150 Protein (4330-SL)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9QUM4

Applications

Bioactivity

View all SLAM/CD150 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SLAM/CD150 protein
Thr25-Pro242, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr25

Predicted Molecular Mass

25 kDa

SDS-PAGE

41-55 kDa, reducing conditions

Activity

Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3.
The ED₅₀ for this effect is 0.6-2.4 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Reviewed Applications

Read 1 review rated 4 using 4330-SL in the following applications:

Characterization prior to use. (1 Review)

Formulation, Preparation, and Storage

4330-SL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mature mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (3). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2-7). It is up‑regulated on activated B cells and CD4⁺ and CD8⁺T cells, although it is down‑regulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8⁺ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, NKT cell development, and the microbicidal response of macrophages to Gram negative bacteria (8, 12-14). In humans, SLAM functions as a cellular entry receptor for measles virus (15, 16).