Recombinant Mouse SLAM/CD150 Protein, CF

Catalog # Availability Size / Price Qty
4330-SL-050
Product Details
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Recombinant Mouse SLAM/CD150 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3. The ED50 for this effect is 0.6-2.4 μg/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse SLAM/CD150 protein
Thr25-Pro242, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr25
Predicted Molecular Mass
25 kDa
SDS-PAGE
41-55 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4330-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: SLAM/CD150

The type I transmembrane glycoprotein Signaling Lymphocytic Activation Molecule (SLAM), also known as CD150, is the prototypic member of the SLAM subgroup of the CD2 protein family. CD2 family proteins function as adhesion molecules and modulators of the immune response (1). Mature mouse SLAM consists of a 218 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 78 aa cytoplasmic domain with three immunoreceptor tyrosine switch motifs (ITSM) (2). Within the ECD, mouse SLAM shares 58% and 83% aa sequence identity with human and rat SLAM, respectively. Alternate splicing generates an isoform with a substituted cytoplasmic domain (2). It is expressed as a 75 kDa molecule of which approximately 30 kDa is N-linked carbohydrate (3). SLAM is expressed on T cells, B cells, thymocytes, macrophages, dendritic cells, platelets, and hematopoietic stem cells (2-7). It is up‑regulated on activated B cells and CD4+ and CD8+ T cells, although it is down‑regulated on Th2 polarized cells (2, 3, 8). SLAM interacts homophilically with low affinity, and this interaction induces a Th0/Th1 response characterized by clonal expansion, production of IFN-gamma, and increased cytolytic activity of CD8+ T cells (2, 3, 9‑11). SLAM ligation also promotes B cell activation, allergen-induced eosinophil and mast cell activation, NKT cell development, and the microbicidal response of macrophages to Gram negative bacteria (8, 12-14). In humans, SLAM functions as a cellular entry receptor for measles virus (15, 16).

References
  1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
  2. Castro, A.G. et al. (1999) J. Immunol. 163:5860.
  3. Cocks, B.G. et al. (1995) Nature 376:260.
  4. Wang, N. et al. (2004) J. Exp. Med. 199:1255.
  5. Hahm, B. et al. (2004) Virology 323:292.
  6. Nanda, N. et al. (2005) Blood 106:3028.
  7. Kiel, M.J. et al. (2005) Cell 121:1109.
  8. Punnonen, J. et al. (1997) J. Exp. Med. 185:993.
  9. Mavaddat, N. et al. (2000) J. Biol. Chem. 275:28100.
  10. Aversa, G. et al. (1997) J. Immunol. 158:4036.
  11. Mehrle, S. et al. (2008) Mol. Immunol. 45:796.
  12. Wang, N. et al. (2006) Am. J. Respir. Cell Mol. Biol. 35:206.
  13. Jordan, M.A. et al. (2011) J. Immunol. 186:3953.
  14. Berger, S.B. et al. (2010) Nat. Immunol. 11:920.
  15. Tatsuo, H. et al. (2000) Nature 406:893.
  16. Hsu, E.C. et al. (2001) Virology 279:9.
Long Name
Signaling Lymphocytic Activation Molecule
Entrez Gene IDs
6504 (Human); 27218 (Mouse)
Alternate Names
CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1

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Recombinant Mouse SLAM/CD150 Protein, CF
By Anonymous on 01/08/2018
Application: Characterization prior to use.