Recombinant Mouse Slit2 (aa 26-900) Protein, CF

Catalog # Availability Size / Price Qty
5444-SL-050
Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (2)

Recombinant Mouse Slit2 (aa 26-900) Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Recombinant Mouse Slit2 immobilized at 3-12 μg/mL, is able to significantly induce neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Slit2 protein
Gln26-Gln900, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained; Gln26 predicted
Predicted Molecular Mass
99.7 kDa
SDS-PAGE
105-115 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5444-SL

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Slit2

Slit Homolog 2 (Slit2) is a member of the Slit family of secreted extracellular matrix glycoproteins that are best known for their role in axon guidance (1). It is expressed in the developing and adult brain and spinal cord, as well as in fetal lung and kidney, and the adult adrenal gland, thyroid gland, and trachea (1-3). Slit2 is composed of multiple domains including nine EGF-like domains, 20 Leucine-rich repeats (LRRs), one Laminin G-like domain, one C-terminal cysteine knot-like (CTCK) domain, and 4 N-terminal and 4 C-terminal LRR domains (1, 3). Slit2 has a molecular weight of approximately 200 kDa (4). However, proteolytic cleavage between the fifth and sixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein, termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5). Mature mouse Slit2 shares 96% and 98% amino acid sequence identity with the human and rat orthologs, respectively.

Slit2 has been shown to bind to multiple receptors including ROBO-1, -2, -3, and -4, Laminin-1, DAN, Gremlin, and Glypican-1 (1, 6-8). Depending upon the target, Slit2 can promote a number of diverse effects. Slit2 regulates axon guidance by binding to ROBO receptors and initiating axon repulsion (1, 5, 9-12). Slit2 has also been shown to induce growth cone collapse, inhibit oligodendrocyte precursor cell migration, and promote axon elongation, branch formation, and fasciculation (5, 13-16). Additionally, Slit2-N and Slit2-C have been shown to have distinct activities. Slit2-N binds to ROBO-1 and repels motor axon migration, while Slit2-C binds to
Glypican-1 and promotes motor axon migration (5). Outside the nervous system, Slit2 plays a role in a wide range of biological processes including cell adhesion and migration, tumor progression and metastasis, angiogenesis, lymphangiogenesis, HIV-1 replication, platelet function and thrombus formation, and stem cell senescence (1, 8, 17-30).

References
  1. Ypsilanti, A.R. et al. (2010) Development 137:1939.
  2. Itoh, A. et al. (1998) Brain Res. Mol. Brain Res. 62:175.
  3. Holmes, G.P. et al. (1998) Mech. Dev. 79:57.
  4. Chédotal, A. (2007) Adv. Exp. Med. Biol. 621:65.
  5. Nguyen Ba-Charvet, K.T. et al. (2001) J. Neurosci. 21:4281.
  6. Nguyen Ba-Charvet, K.T. et al. (2001) Mol. Cell. Neurosci. 17:1048.
  7. Hagino, S. et al. (2003) Glia 42:130.
  8. Chen, B. et al. (2004) J. Immunol. 173:5914.
  9. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  10. Erskine, L. et al. (2000) J. Neurosci. 20:4975.
  11. Shu, T. et al. (2003) J. Neurosci. 23:8176.
  12. Kim, M. et al. (2014) Neural Dev. 9:17.
  13. Wang, K.H. et al. (1999) Cell 96:771.
  14. Wong, E.V. et al. (2004) J. Neurobiol. 59:66.
  15. Liu, X. et al. (2012) J. Biol. Chem. 287:17503.
  16. Jaworski, A. et al. (2012) Nat. Neurosci. 15:367.
  17. Guan, H. et al. (2003) J. Immunol. 171:6519.
  18. Liu, D. et al. (2006) Circ. Res. 98:480.
  19. Prasad, A. et al. (2007) J. Leukoc. Biol. 82:465.
  20. Tole, S. et al. (2009) J. Leukoc. Biol. 86:1403.
  21. Kim, H.K. et al. (2008) Neoplasia 10:1411.
  22. Prasad, A. et al. (2008) J. Biol. Chem. 283:26624.
  23. Tseng, R.C. et al. (2010) Cancer Res. 70:543.
  24. Alajez, N.M. et al. (2011) Cancer Res. 71:2381.
  25. Zhang, Q.Q. et al. (2015) Oncotarget 6:3123.
  26. Dunaway, C.M. et al. (2011) Mol. Cell. Biol. 31:404.
  27. Yang, X.M. et al. (2010) Biochem. Biophys. Res. Commun. 396:571.
  28. Anand, A.R. et al. (2011) AIDS 25:2105.
  29. Patel, S. et al. (2012) Circulation 126:1385.
  30. Harburg, G. et al. (2014) Stem Cell Reports 3:385.
Long Name
SLIT Homolog 2
Entrez Gene IDs
9353 (Human); 20563 (Mouse); 360272 (Rat)
Alternate Names
FLJ14420; SLIL3; slit (Drosophila) homolog 2; slit homolog 2 (Drosophila); slit homolog 2 protein; Slit2; Slit-2SLIL3

Citations for Recombinant Mouse Slit2 (aa 26-900) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis
    Authors: G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann
    Nat Commun, 2019;10(1):2350.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Different Isoforms of the Neuronal Guidance Molecule Slit2 Directly Cause Chemoattraction or Chemorepulsion of Human Neutrophils
    Authors: D Pilling, LE Chinea, KM Consalvo, RH Gomer
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Robo signalling controls pancreatic progenitor identity by regulating Tead transcription factors
    Authors: S Escot, D Willnow, H Naumann, S Di Frances, FM Spagnoli
    Nat Commun, 2018;9(1):5082.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  4. The microtubule plus-end-tracking protein TACC3 promotes persistent axon outgrowth and mediates responses to axon guidance signals during development
    Authors: B Erdogan, GM Cammarata, EJ Lee, BC Pratt, AF Francl, EL Rutherford, LA Lowery
    Neural Dev, 2017;12(1):3.
    Species: Xenopus
    Sample Types: Neural Tube Explants
    Applications: Bioassay
  5. Robo1 Forms a Compact Dimer-of-Dimers Assembly
    Authors: N Aleksandro, I Gutsche, E Kandiah, SV Avilov, MV Petoukhov, E Seiradake, AA McCarthy
    Structure, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Proximity Ligation Assay
  6. The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization
    Nat Commun, 2016;7(0):13517.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Fibroblasts secrete Slit2 to inhibit fibrocyte differentiation and fibrosis.
    Authors: Pilling D, Zheng Z, Vakil V, Gomer R
    Proc Natl Acad Sci U S A, 2014;111(51):18291-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Robo1 modulates proliferation and neurogenesis in the developing neocortex.
    Authors: Yeh M, Gonda Y, Mommersteeg M, Barber M, Ypsilanti A, Hanashima C, Parnavelas J, Andrews W
    J Neurosci, 2014;34(16):5717-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
    Authors: Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
    Exp. Cell Res., 2012;318(4):371-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  10. Angiocrine factors modulate tumor proliferation and motility through EphA2 repression of Slit2 tumor suppressor function in endothelium.
    Authors: Brantley-Sieders DM, Dunaway CM, Rao M
    Cancer Res., 2011;71(3):976-87.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Activation of endothelial roundabout receptor 4 reduces the severity of virus-induced keratitis.
    Authors: Mulik S, Sharma S, Suryawanshi A
    J. Immunol., 2011;186(12):7195-204.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis.
    Authors: Dunaway CM, Hwang Y, Lindsley CW
    Mol. Cell. Biol., 2011;31(3):404-16.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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Reviews for Recombinant Mouse Slit2 (aa 26-900) Protein, CF

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Recombinant Mouse Slit2 (aa 26-900) Protein, CF
By Anonymous on 10/04/2019
Application: Immunoassay Standard

We used this protein for a sandwich ELISA format in combination with mAb (5444)) and pAb (AF5444). This combination works well for detecting SLIT2 in human serum.


Recombinant Mouse Slit2 Protein, CF
By Anonymous on 08/08/2016
Application: Cell migration/motility
Reason for Rating: We used this product to check effect of Slit-2 protein on migration of murine Lin negative- C-kit positive cells.