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Recombinant Mouse TAPBPR Fc Chimera Protein, CF

Catalog #: 11254-TP Datasheet / COA / SDS
Analyzed by SEC-MALS
Catalog # Availability Size / Price Qty
11254-TP-050

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Recombinant Mouse TAPBPR Fc Chimera Protein SEC-MALS.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse TAPBPR Fc Chimera Protein, CF Summary

  • R&D Systems NS0-derived Recombinant Mouse TAPBPR Fc Chimera Protein (11254-TP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 0.200‑5.00 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse TAPBPR protein
Mouse TAPBPR
(Thr21-Gly412)
Accession # NP_663366.2		IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
NP_663366.2
N-terminal Sequence
Analysis
Thr21
Predicted Molecular Mass
69 kDa
SDS-PAGE
75-90 kDa, under reducing conditions.

Product Datasheets

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11254-TP

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11254-TP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Mouse TAPBPR Fc Chimera Protein (Catalog # 11254-TP) has a molecular weight (MW) of 148.5 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Bioactivity View Larger

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 0.200‑5.00 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse TAPBPR Fc Chimera Protein (Catalog # 11254-TP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-90 kDa and 150-180 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAPBPR

TAP-binding protein-like (TAPBPL), also known as TAP binding protein-related (TAPBPR) and Tapasin-related protein (TAPASINR) is a transmembrane protein of the Immunoglobulin (Ig) superfamily (1, 2). TAPBPR was originally isolated as a homologue to TAPASIN but more recently was identified as a novel B7 family-related molecule since it shares sequence, structural, and functional similarities to B7 family members (3). Mature mouse TAPBPR consists of a lumenal domain containing an IgV and IgC domain, a transmembrane domain, and a cytoplasmic tail.  Within the lumenal domain, mature mouse TAPBPR shares 70% and 87% amino acid sequence identity with human and rat TAPBPR, respectively. Multiple alternatively spliced TAPBPR isoforms are known to exist with unique properties (4).TAPBPR is widely expressed and, similar to TAPASIN, functions as a both a chaperone protein and peptide editor of MHC class I, but in a peptide‑loading complex (PLC) independent manner (5, 6). TAPBPR decreases the rate at which MHC class I molecules mature through the secretory pathway, a role which could be important for peptide selection by MHC class I molecules (7). TAPBPR is also expressed on the surface of T cells and antigen-presenting cells (APCs) and plays an inhibitory role in the proliferation and activation of T cells (4). TAPBPR can be expressed on various cancer cells like leukemia and has the potential to be used in the treatment of autoimmune diseases and transplant rejection, as well as cancer (4).

References
  1. Hermann, C. et al. (2015) Tissue antigens 85(3):155.
  2. Teng, M. et al. (2002) European Journal of Immunology 32:1059.
  3. Lin, Y. et al. (2021). EMBO Mol Med. 13(5):13404.
  4. Porter, K.M. et al. (2014) Immunology 142:289.
  5. Margulies, D. et al. (2020) Current Opinion in Immunology 64:71.
  6. Boyle, L.H. et al. (2013) PNAS 110:3465.
  7. Hermann, C. et al. (2013) Journal of Immunology 191(11):5743.
Long Name
Tapasin Related Protein
Entrez Gene IDs
55080 (Human); 213233 (Mouse); 297602 (Rat)
Alternate Names
FLJ10143; TAP binding protein-like; Tapasin Related Protein/TAPBPR; Tapasin-like; TAPASIN-R; tapasin-related protein; TAP-binding protein-like; TAP-binding protein-related protein; TAPBPL; TAPBPR; TAPBP-RTAP binding protein related; TAPBPRtapasin-like

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