Recombinant Mouse Thrombospondin-1 Protein, CF
Recombinant Mouse Thrombospondin-1 Protein, CF Summary
Accession # NP_035710
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Thrombospondin‑1 (TSP‑1) is a 150‑180 kDa calcium‑sensitive protein that is secreted as a disulfide‑linked homotrimer. Within the Thrombospondin family, TSP‑2 is also homotrimeric, while TSP-3, -4, and -5/COMP are pentameric. TSP‑1 regulates a wide range of cellular functions including their interactions with other cells and with the extracellular matrix (ECM). TSP-1 contains an N‑terminal Laminin G‑like globular domain, an extended central region with one vWF‑C domain, 3 TSP type 1 repeats, 3 EGF‑like domains, and 8 TSP type‑3 repeats, and a globular C‑terminal lectin domain. Distinct regions of TSP‑1 have been associated with binding to particular ECM or cellular molecules (1, 2). Mature mouse TSP‑1 shares 95% and 99% amino acid sequence identity with human and rat TSP‑1, respectively. TSP‑1 counteracts the angiogenic, hypotensive, and anti‑thrombotic effects of nitric oxide (NO) (3). It binds and neutralizes VEGF, blocks VEGF R2 signaling on vascular endothelial cells (EC), and destabilizes adhesive contacts between EC (4‑6). TSP‑1’s actions on vascular smooth muscle cells support arterial tone and blood pressure in the presence of vasodilatory stimulation (7). It is released from activated platelets and enhances platelet aggregation and adhesion (8). TSP-1 also plays an important role in wound repair and tissue fibrosis by binding latent TGF‑beta and inducing release of the active cytokine from the latency associated peptide (LAP) (9, 10). TSP‑1 dampens adaptive immune responses by inducing the differentiation of regulatory T cells and inhibiting TCR signaling (11, 12). In the nervous system, it promotes excitatory synapse formation (13, 14) and supports the integration of neural progenitor cells into the olfactory bulb (15). In addition, TSP‑1 is released by apoptotic cells and promotes macrophage-mediated debris clearance (16).
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- Sweetwyne, M.T. and J.E. Murphy-Ullrich (2012) Matrix Biol. 31:178.
- Grimbert, P. et al. (2006) J. Immunol. 177:3534.
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- Xu, J. et al. (2010) Nat. Neurosci. 13:22.
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Citations for Recombinant Mouse Thrombospondin-1 Protein, CF
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Citations: Showing 1 - 3
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Embryonic Stem Cell-Derived Peripheral Auditory Neurons Form Neuralï¿½Connections with Mouse Central Auditory Neurons Inï¿½Vitro via theï¿½?2?1 Receptor
Authors: Z Liu, Y Jiang, X Li, Z Hu
Stem Cell Reports, 2018;0(0):.
Sample Types: Whole Cells
Thrombospondin-1 signaling through CD47 inhibits cell cycle progression and induces senescence in endothelial cells
Cell Death Dis, 2016;7(9):e2368.
Sample Types: Whole Cells
Photodynamic therapy-mediated cancer vaccination enhances stem-like phenotype and immune escape, which can be blocked by thrombospondin-1 signaling through CD47 receptor protein.
Authors: Zheng Y, Zou F, Wang J, Yin G, Le V, Fei Z, Liu J
J Biol Chem, 2015;290(14):8975-86.
Sample Types: Whole Cells
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