Recombinant Mouse Thrombospondin-4 Protein, CF

Catalog # Availability Size / Price Qty
7860-TH-050
Product Details
Citations (3)
FAQs
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Recombinant Mouse Thrombospondin-4 Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 0.1-0.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Thrombospondin-4 protein
Gln27-Asn963, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gln27 predicted: No results obtained, sequencing might be blocked
Predicted Molecular Mass
104.4 kDa
SDS-PAGE
120-145 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7860-TH

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Thrombospondin-4

Thrombospondin-4 (TSP-4) is an approximately 140 kDa matricellular protein that is secreted as a disulfide-linked pentamer. Within the Thrombospondin family, TSP‑3 and TSP‑5/COMP are also pentameric, while TSP‑1 and TSP‑2 are trimeric. TSP‑4 regulates cell‑cell and cell matrix interactions and plays a role in cardiovascular physiology and neuronal development (1, 2). Mature mouse TSP‑4 consists of an N‑terminal heparin-binding domain, a coiled coil motif, four EGF‑like repeats, seven TSP type‑3 repeats (one with an RGD motif), and a TSP C‑terminal domain (2, 3). Mouse TSP‑4 shares 93% and 97% amino acid sequence identity with human and rat TSP‑4, respectively. TSP‑4 binds a variety of matrix proteins including Collagens I, II, III, V, Laminin‑1, Fibronectin, and Matrilin‑2 (4). Interactions of TSP‑4 with non‑collagenous proteins are independent of divalent cations, whereas interactions with collagenous proteins are enhanced in the presence of zinc (4). TSP‑4 binds to cell surface Integrins containing the alpha M, beta 2, or beta 3 chains (5, 6). It is expressed in skeletal muscle and tendon as well as by vascular smooth muscle and endothelial cells (7‑9). It is up‑regulated in cardiomyocytes during pressure overload and is required for mediating the responsive increase in cardiac contractility (10). In humans, a polymorphism of TSP-4 (A387P) is associated with myocardial infarction (11). TSP-4 contributes to the development of inflammation and atherosclerosis by promoting macrophage and neutrophil adhesion to the vasculature (5, 6). In the nervous system, TSP‑4 is expressed by astrocytes and neurons and is enriched at neuromuscular junctions and synapse‑rich layers of the brain and retina (9, 12, 13). It promotes neuronal adhesion, neurite outgrowth, and excitatory synaptogenesis (9, 13, 14). TSP‑4 is up‑regulated in the spinal cord following peripheral nerve injury where it contributes to presynaptic hypersensitivity and hyperalgesia (12). It is also up‑regulated in muscle following denervation (9). TSP‑4 is additionally secreted by tumor‑associated fibroblasts in gastric adenocarcinoma, particularly in regions of tumor cell invasion (15).

References
  1. Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
  2. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
  3. Newton, G. et al. (1999) Mamm. Genome 10:1010.
  4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
  5. Pluskota, E. et al. (2005) Blood 106:3970.
  6. Frolova, E.G. et al. (2010) Circ. Res. 107:1313.
  7. Sodersten, F. et al. (2007) Connect. Tiss. Res. 48:254.
  8. Stenina, O.I. et al. (2003) Circulation 108:1514.
  9. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
  10. Cingolani, O.H. et al. (2011) Circ. Res. 109:1410.
  11. Stenina, O.I. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:1886.
  12. Kim, D.-S. et al. (2012) J. Neurosci. 32:8977.
  13. Dunkle, E.T. et al. (2007) Exp. Eye Res. 84:707.
  14. Eroglu, C. et al. (2009) Cell 139:380.
  15. Forster, S. et al. (2011) Mod. Pathol. 24:1390.
Entrez Gene IDs
7060 (Human); 21828 (Mouse)
Alternate Names
THBS4; thrombospondin 4; Thrombospondin4; Thrombospondin-4; TSP4; TSP-4

Citations for Recombinant Mouse Thrombospondin-4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Thrombospondin-4 induces prolongation of action potential duration in rat isolated ventricular myocytes
    Authors: K Imoto, M Aratani, T Koyama, M Okada, H Yamawaki
    J. Vet. Med. Sci., 2020;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Thrombospondin-3 augments injury-induced cardiomyopathy by intracellular integrin inhibition and sarcolemmal instability
    Authors: TG Schips, D Vanhoutte, A Vo, RN Correll, MJ Brody, H Khalil, J Karch, A Tjondrokoe, MA Sargent, M Maillet, RS Ross, JD Molkentin
    Nat Commun, 2019;10(1):76.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Thrombospondin expression in myofibers stabilizes muscle membranes
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Types:
    Applications: Bioassay

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