Recombinant Mouse TLR4 Fc Chimera Protein, CF

  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse TLR4 Fc Chimera is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human MD-2 (Catalog # 1787-MD) that produces 50% of the optimal binding response is approximately 0.15-0.9 μg/mL
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Mouse TLR4
    Accession # Q9QUK6
    DIEGRMDP Mouse IgG2a
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    96 kDa
    115-137 kDa, reducing conditions
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Mouse TLR4 Fc Chimera (Catalog # 9149-TR) is coated at 2 µg/mL (100 μL/well), Recombinant Human MD-2 (Catalog #
1787-MD) binds with a typical ED50 of 0.15-0.9 μg/mL.
Background: TLR4
TLR4, also known as CD284, is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules. The complex of TLR4 with MD-2 functions as a critical receptor for bacterial endotoxin/lipopolysaccharide (LPS) (1-3). Mature mouse TLR4 consists of a 613 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 176 aa cytoplasmic domain. TLR4 contains 19 leucine rich repeats in its ECD and one cytoplasmic Toll/IL-1 receptor (TIR) domain (4-6). Within the ECD, mouse TLR4 shares 63% and 83% aa sequence identity with human and rat TLR4, respectively. On monocytes, macrophages, dendritic cells, and B cells, MD‑2 expression is required for cell surface localization of TLR4 and for optimal LPS‑induced TLR4 signaling (7-10). MD‑2 also forms soluble disulfide-linked homo-oligomers which can interact with TLR4 (8). Through a domain separate from its TLR4-binding domain, MD‑2 extracts LPS from circulating CD14‑LPS complexes and carries the LPS into a ternary complex with TLR4 (11-13). The interaction of
MD‑2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (2, 14). Increased levels of plasma MD‑2 in septic shock patients sensitizes MD‑2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (15).
  • References:
    1. Mukherjee, S. et al. (2016) Braz. J. Infect. Dis. 20:193.
    2. Plociennikowska, A. et al. (2015) Cell. Mol. Life Sci. 72:557.
    3. Erridge, C. (2010) J. Leukoc. Biol. 87:989.
    4. Medzhitov, R. et al. (1997) Nature 388:394.
    5. Poltorak, A. et al. (1998) Science 282:2085.
    6. Qureshi, S.T. et al. (1999) J. Exp. Med. 189:615.
    7. Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
    8. Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12156.
    9. Akashi, S. et al. (2000) J. Immunol. 164:3471.
    10. Nagai, Y. et al. (2002) Nat. Immunol. 3:667.
    11. Re, F. and J.L. Strominger (2003) J. Immunol. 171:5272.
    12. Kennedy, M.N. et al. (2004) J. Biol. Chem. 279:34698.
    13. Gioannini, T.L. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4186.
    14. Saitoh, S. et al. (2004) J. Endotoxin Res. 10:257.
    15. Pugin, J. et al. (2004) Blood 104:4071.
  • Long Name:
    Toll-like Receptor 4
  • Entrez Gene IDs:
    7099 (Human); 21898 (Mouse); 29260 (Rat)
  • Alternate Names:
    ARMD10; CD284 antigen; CD284; hTollhomolog of Drosophila toll; TOLL; toll-like receptor 4
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