Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF Summary
Ala20-Glu762, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Mouse VEGFR2/KDR/Flk-1 (Catalog # 10095-KD) is immobilized at 0.5 μg/mL, 100 μL/well, Recombinant Mouse VEGF 164 (Catalog # 493-MV) binds with an ED50 of 0.015‑0.12 μg/mL.
2 μg/lane of Recombinant Mouse VEGFR2/KDR/Flk‑1 His-tag (Catalog # 10095-KD) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 120-132 kDa.
VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and a tyrosine kinase domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature mouse VEGFR2 is composed of a 743aa residue extracellular domain, a 21 aa residue transmembrane domain and a 584 aa residue cytoplasmic domain. The mature mouse VEGFR2 shares 80% and 93% sequence identity to human and rat VEGFR2. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2 binds VEGF with high affinity and is a potent VEGF antagonist (1, 2).
- Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
- Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.
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