Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10095-KD-050
Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF Binding Activity
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Recombinant Mouse VEGFR2/Flk-1 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse VEGFR2/KDR/Flk-1 His-tag (Catalog # 10095-KD) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Recombinant Mouse VEGF 164 (Catalog # 493-MV) that produces 50% of the optimal binding response is 0.015-0.12 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse VEGFR2/KDR/Flk-1 protein
Ala20-Glu762, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala20
Structure / Form
Monomer
Predicted Molecular Mass
844 kDa
SDS-PAGE
120-132 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10095-KD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Mouse VEGFR2/KDR/Flk-1 (Catalog # 10095-KD) is immobilized at 0.5 μg/mL, 100 μL/well, Recombinant Mouse VEGF 164 (Catalog # 493-MV) binds with an ED50 of 0.015‑0.12 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse VEGFR2/KDR/Flk‑1 His-tag (Catalog # 10095-KD) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 120-132 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and a tyrosine kinase domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature mouse VEGFR2 is composed of a 743aa residue extracellular domain, a 21 aa residue transmembrane domain and a 584 aa residue cytoplasmic domain. The mature mouse VEGFR2 shares 80% and 93% sequence identity to human and rat VEGFR2. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2 binds VEGF with high affinity and is a potent VEGF antagonist (1, 2).

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
  2. Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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