Recombinant Mouse VLDLR (Cys297) Protein, CF

Catalog # Availability Size / Price Qty
6685-VL-050
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Mouse VLDLR (Cys297) Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

When Recombinant Human Apolipoprotein E3 (Catalog # 4144-AE) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse VLDL R that produces 50% of the optimal binding response is found to be approximately 0.075 ‑ 0.375 μg/mL.

This protein has also been shown to bind to Recombinant Mouse LRPAP (Catalog # 4480-LR) and Recombinant Mouse Reelin (Catalog # 3820-MR).

Source
Mouse myeloma cell line, NS0-derived mouse VLDL R protein
Met1-Ala798, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Thr25
Predicted Molecular Mass
86.5 kDa
SDS-PAGE
110-135 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6685-VL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: VLDLR

VLDL R is a 130 kDa type I transmembrane protein in the LDL receptor family that plays a significant role in lipid metabolism and in nervous system development and function (1, 2). Mouse VLDL R has a 774 aa extracellular domain (ECD) (aa 25 ‑ 798) and a 54 aa cytoplasmic domain. The ECD contains eight LDLR class A repeats, three EGF‑like domains, six LDLR class B repeats, and a juxtamembrane region that is rich in O‑linked glycosylation (3, 4, 5). The cytoplasmic domain contains one NPXY internalization motif. Mouse VLDL R has at least one 105 kDa alternative splice form. This variant (termed type II VLDL R) shows an Arg substitution for aa 751 ‑ 779, and has been associated with endothelial cells (5, 6). The VLDL R expressed here corresponds to a polymorphic form that shows a Cys at position 297 of the precursor. This generates a disulfide bond in the 7th LDL class A domain that is not present when position 297 is occupied by Ser (SwissProt # P98156). The 7th domain is suggested to be a critical determinant of apoE binding to VLDL R (7). VLDL R is predominantly expressed in striated muscle, adipose tissue, brain, and endothelial cells lining capillaries and small arterioles (3, 4, 8, 9). VLDL R participates in the tissue uptake of fatty acids from plasma by mediating the internalization of ApoE‑containing lipoparticles (i.e. VLDL, beta ‑VLDL, and chylomicron remnants) (8, 10). VLDL R binds and internalizes lipoprotein lipase (LPL) and mediates its transport from the basolateral to the lumenal face of endothelial cells (9, 11). VLDL R knockout mice are characterized by reduced LPL activity, reduced serum triglyceride clearance, and a resistance to developing obesity (10, 12, 13). VLDL R influences breast cancer cell motility by mediating the uptake of uPAR‑PAI1 complexes (9, 14). Lipoprotein accumulation via macrophage VLDL R is instrumental in promoting the formation of atherosclerotic plaques (15). In the nervous system, VLDL R and ApoE R2 interactions with reelin are critical for neuronal migration and positioning in the developing brain (16). VLDL R also functions in adult hippocampal synapse maturation, synaptic plasticity, and memory formation (17, 18). The ECD of mouse VLDL R shares 95% aa sequence identity with human and rat VLDL R. Within shared regions, mouse VLDL R shares 55% and 53% aa sequence identity with ApoE R2 and LDL R, respectively.

References
  1. Qiu, S. et al. (2006) Neurobiol. Learn. Mem. 85:16.
  2. May, P. et al. (2005) Cell. Mol. Life Sci. 62:2325.
  3. Gafvels, M.E. et al. (1994) Endocrinology 135:387.
  4. Oka, K. et al. (1994) Eur. J. Biochem. 224:975.
  5. Martensen, P.M. et al. (1997) Eur. J. Biochem. 248:583.
  6. GenBank Accession # NP_001154892.
  7. Ruiz, J. et al. (2005) J. Lipid Res. 46:1721.
  8. Wyne, K.L. et al. (1996) Arterioscler. Thromb. Vasc. Biol. 16:407.
  9. Argraves, K.M. et al. (1995) J. Biol. Chem. 270:26550.
  10. Goudriaan, J.R. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1488.
  11. Obunike, J.C. et al. (2001) J. Biol. Chem. 276:8934.
  12. Yagyu, H. et al. (2002) J. Biol. Chem. 277:10037.
  13. Goudriaan, J.R. et al. (2004) J. Lipid Res. 45:1475.
  14. Webb, D.J. et al. (1999) J. Biol. Chem. 274:7412.
  15. van Eck, M. et al. (2005) Atherosclerosis 183:230.
  16. Jossin, Y. et al. (2004) J. Neurosci. 24:514.
  17. Niu, S. et al. (2004) Neuron 41:71.
  18. Weeber, E.J. et al. (2002) J. Biol. Chem. 277:39944.
Long Name
Very Low Density Lipoprotein Receptor
Entrez Gene IDs
7436 (Human); 22359 (Mouse)
Alternate Names
CARMQ1; CHRMQ1; FLJ35024; very low density lipoprotein receptor; very low-density lipoprotein receptor; VLDL R; VLDL receptor; VLDLR; VLDL-R; VLDLRCH

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse VLDLR (Cys297) Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse VLDLR (Cys297) Protein, CF and earn rewards!

Have you used Recombinant Mouse VLDLR (Cys297) Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review