Recombinant Mouse VSIG4 Fc Chimera Protein, CF Summary
Why choose R&D Systems VSIG-4 Protein?
- Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
- Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
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The non-Fc, His-tagged mouse VSIG-4 is available here Mouse VSIG-4 His Protein
Accession # NP_808457
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse VSIG4 Fc Chimera (Catalog # 4674-VS) inhibits anti-CD3-induced proliferation of mouse splenic T lymphocytes. The presence of immobilized Recombinant Mouse VSIG4 Fc Chimera inhibits the anti-CD3 response by ≥50%. The ED50 for this effect is 1.25‑5 µg/mL.
Mouse VSIG4 (V-set and immunoglobulin domain containing 4), also known as CRIg and Z39IG, is a 45 kDa, type I transmembrane glycoprotein that is a B7 family-related protein and an Ig superfamily member (1, 2). Mouse VSIG4 is synthesized as a 280 amino acid (aa) precursor that contains a signal sequence, an IgV-type immunological domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain (2). The IgV domain of mouse VSIG4 shares 86% and 80% aa sequence identity with the IgV domains of rat and human VSIG4, respectively. Quantitative PCR reveals that VSIG4 mRNA is expressed at high levels in the liver, dendritic cells, neutrophils, and macrophages, and at lower levels in the lung, heart, spleen, and lymph nodes (1). No VSIG4 expression appears to be present in T and B cells (1). The use of polyclonal rabbit serum against the extracellular domain of VSIG4 demonstrates that VSIG4 is specifically expressed on naïve resting tissue macrophages, and that the expression is downregulated or lost upon activation (1). Furthermore, histological analysis shows expression of VSIG4+ macrophages only in the liver, thymic medulla, and heart (1). VSIG4 macrophages are not detected in the intestines, kidney, skeletal muscle, lymph node, splenic white pulp, lung, or brain (1). Studies show that VSIG4/Fc Chimera strongly inhibits proliferation of anti-CD3 as well as anti-CD3/anti-CD28-stimulated T cells (1). Indeed, VSIG4 functions as a negative regulator of mouse as well as human T cell activation, and may be involved in the maintenance of peripheral T cell tolerance and/or unresponsiveness (1). In addition, VSIG4’s expression on Kupffer cells is required for efficient binding and phagocytosis of complement C3 opsonized particles (2). VSIG4 acts as a macrophage complement receptor by binding complement fragments C3b and iC3b (2). VSIG4 binding to C3b inhibits complement activation through the alternative pathway, making it a potent suppressor of established inflammation (3, 4).
- Vogt, L. et al. (2006) J. Clin. Invest. 116:2817.
- Helmy, K. et al. (2006) Cell 124:915.
- Katschke, K.J. et al. (2007) J. Exp. Med. 204:1319.
- Wiesmann, C. et al. (2006) Nature 444:217.
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