Recombinant Mouse VSTM4 His-tag Protein, CF Summary
Leu24-Tyr179, with C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse VSTM4 (Catalog # 10210-VT) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-5 μg/mL.
2 μg/lane of Recombinant Mouse VSTM4 His-tag (Catalog # 10210-VT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-44 kDa.
V-set and transmembrane domain-containing protein 4 (VSTM4) is a single-pass type I membrane protein in the immunoglobulin superfamily. Mouse VSTM4 is synthesized as a 319 amino acid (aa) precursor that contains a 23 aa signal sequence, a 156 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 119 aa cytoplasmic tail. In humans, part of the extracellular region is cleaved into a 50 aa secreted peptide (aa 55-104) compared to mouse, which is cleaved into a 49 aa peptide (aa 55-103) (1). Because of its role in enhancing L-type voltage-gated calcium channel (L-VGCC) currents in photoreceptors, this peptide was named peptide Lv (1). Peptide Lv is expressed in the central nervous system and a variety of organs including spleen, intestine, retina, and lung (1, 2). The peptide may have possible roles in regulating the cardiovascular system and L-VGCC dependent neural plasticity (1, 2). Human VSTM4 gene is located on chromosome 10, which may be linked to late-onset Alzheimer's disease (3). Down-regulation of VSTM4 increased tamoxifen sensitivity and suppressed growth in cultured breast cancer cells (4). Within the ECD, mouse VSTM4 shares 87% and 96% aa sequence identity with human and rat VSTM4, respectively. The biological functions of VSTM4 remain unknown. Our in-house data show that mouse VSTM4 inhibits anti-CD3 induced IL-2 secretion by Human T Cells.
- Shi, L. et al. (2012) PLoS. 7:e43091.
- Shi, L. et al. (2015) Biochim. Biophys. Acta. 1853:1154.
- Grupe, A. et al. (2006) Am. J. Hum. Genet. 78:78.
- Mendes-Pereira, A. et al. (2012) Proc. Natl. Acad. Sci. 109:2730.
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