Recombinant Mouse WIF-1 Protein, CF

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135-WF-050

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Recombinant Mouse WIF-1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.7-3.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse WIF-1 protein
Mouse WIF-1
(Gly29-Trp379)
Accession # Q9WUA1
HIEGR GGGSGGGSGGGS  10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gly29
Predicted Molecular Mass
41.1 kDa
SDS-PAGE
50-55 kDa, reducing conditions

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135-WF

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

135-WF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid, NaCl, PEG3350 and CHAPS, pH5.0.
Reconstitution Reconstitute at 500 μg/mL in sterile, deionized water.  .
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: WIF-1

WIF-1 (Wnt Inhibitory Factor-1) is a secreted Wnt inhibitor. Like sFRPs (secreted Frizzled-related proteins), WIF-1 binds to Wnt proteins and inhibits their receptor binding, thus inhibiting all Wnt signaling pathways (1, 2). WIF-1 is synthesized as a 379 amino acid (aa) precursor that contains a 28 aa N-terminal signal sequence, a WIF domain (aa 38-177), 5 EGF-like repeats (aa 178-338), and a C-terminal hydrophilic domain (1). Mature mouse WIF-1 shares 95%, 99%, 87% and 85% aa sequence identity with human, rat, equine and Xenopus WIF-1, respectively. A potential 220 aa isoform with an alternate N-terminus that lacks the WIF domain is identical with the 379 aa form after aa 179 (3).  The WIF domain contains a phospholipid binding site that interacts with lipid-modified Wnt molecules, while a binding site for sulfated proteoglycans within the EGF repeats contributes to binding and inhibition (4). WIF-1 fine-tunes Wnt signaling by time- and location-specific expression in the fetal and postnatal mouse, especially during joint development and cardiomyogenesis (5-7). Binding of Wnts 3a, 4, 5, 7a, 9a and 11, with little or no binding to Wnts 5b, 7b, and 9b, has been shown (5). Binding and inhibition of other molecules in cartilage, such as CTGF (connective tissue growth factor), has also been shown (6). WIF-1 expression is up‑regulated by BMP-4/ Smad1 signaling during fetal lung development (8). WIF-1 functions as a tumor suppressor in cancers of the prostate, breast, lung, bladder, cervix, liver and brain (9-12). Its down‑regulation by promoter hypermethylation is detected in a variety of tumors (11-13). WIF‑1 anti‑tumor effects include antagonizing angiogenesis, growth and invasion, and inducing apoptosis, senescence, or differentiation (2, 10-13). WIF-1 has been used experimentally to antagonize tumors or regulate developmental Wnt activities (10-15).

References
  1. Hsieh, J-C. et al. (1999) Nature 398:431.
  2. Zhang, B. and J. Ma (2010) Protein Cell 1:898.
  3. GenBank Accession # AAH04048.
  4. Malinauskas, T. et al. (2011) Nat. Struct. Mol. Biol. 18:886.
  5. Surmann-Schmitt, C. et al. (2009) J. Cell Sci. 122:3627.
  6. Surmann-Schmitt, C. et al. (2011) J. Cell Physiol. Sept. 16 [Epub ahead of print]
  7. Buermans, H.P.J. et al. (2010) PLoS ONE 5:e15504.
  8. Xu, B. et al. (2011) Development 138:925.
  9. Wissmann, C. et al. (2003) J. Pathol 201:204.
  10. Hu, J. et al. (2009) Cancer Res. 69:6951.
  11. Ramachandran, I. et al. (2011) Oncogene Oct. 17 [Epub ahead of print]
  12. Lambiv, W.L. et al. (2011) Neuro. Oncol. 13:736.
  13. Kansara, M. et al. (2009) J. Clin. Invest. 119:837.
  14. Yee, D.S. et al. (2010) Mol. Cancer 9:162.
  15. Schaniel, C. et al. (2011) Blood 118:2420.
Long Name
Wnt Inhibitory Factor
Entrez Gene IDs
11197 (Human); 24117 (Mouse)
Alternate Names
WIF1; WIF-1; WNT inhibitory factor 1

Citations for Recombinant Mouse WIF-1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Tumor Explants Elucidate a Cascade of Paracrine SHH, WNT, and VEGF Signals Driving Pancreatic Cancer Angiosuppression
    Authors: Hasselluhn, MC;Decker-Farrell, AR;Vlahos, L;Thomas, DH;Curiel-Garcia, A;Maurer, HC;Wasko, UN;Tomassoni, L;Sastra, SA;Palermo, CF;Dalton, TC;Ma, A;Li, F;Tolosa, EJ;Hibshoosh, H;Fernandez-Zapico, ME;Muir, A;Califano, A;Olive, KP;
    Cancer discovery
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Impaired differentiation potential of CD34-positive cells derived from mouse hair follicles after long-term culture
    Authors: Y Ouji, M Misu, T Kitamura, D Okuzaki, M Yoshikawa
    Scientific Reports, 2022-06-30;12(1):11011.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Dysregulation of Wnt inhibitory factor 1 (Wif1) expression resulted in aberrant Wnt-beta-catenin signaling and cell death of the cloaca endoderm, and anorectal malformations.
    Authors: Ng R, Matsumaru D, Ho A, Garcia-Barcelo M, Yuan Z, Smith D, Kodjabachian L, Tam P, Yamada G, Lui V
    Cell Death Differ, 2014-03-14;21(6):978-89.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Endocardial to myocardial notch-wnt-bmp axis regulates early heart valve development.
    Authors: Wang, Yidong, Wu, Bingruo, Chamberlain, Alyssa A, Lui, Wendy, Koirala, Pratisth, Susztak, Katalin, Klein, Diana, Taylor, Verdon, Zhou, Bin
    PLoS ONE, 2013-04-01;8(4):e60244.
    Species: Mouse
    Sample Types: Whole Organism
    Applications: Bioassay

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