Recombinant Mouse WISP-1/CCN4 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse WISP-1/CCN4 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit the cell growth of DU145 human prostate carcinoma cells. The ED50 for this effect is 1.5 -9 µg/mL.
Mouse myeloma cell line, NS0-derived mouse WISP-1/CCN4 protein
Thr23-Asn367, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
39.6 kDa
65 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: WISP-1/CCN4

Mouse WISP-1 (Wnt-induced secreted protein-1; also named CNN4) is a 40 kDa, secreted, heparin-binding glycoprotein that is a member of the CCN (or CTGF/Cyr61/Nov) cysteine-rich protein family (1-6). It is synthesized as a 367 amino acid (aa) precursor that contains a series of structural homology modules. Following a 22 aa signal sequence, there is a 68 aa IGFBP-like domain (aa 53-120), a 66 aa von Willebrand factor type C (VWC) module (aa 121-186), a 41 aa TSP type I domain (aa 220-260) and a 75 aa, C-terminal cysteine knot motif (aa 273-347) (1, 6). The VWC module is associated with protein-protein interaction, the TSP domain binds sulfated glycoconjugates, and the cysteine knot mediates dimerization and receptor binding (4). It is likely that WISP-1 normally circulates as an 80 kDa homodimer (2). Multiple splice forms are reported for human WISP-1, but none for rodent (7-9). Mature mouse WISP-1 is 98%, 84% and 85% aa identical to rat, canine and human WISP-1, respectively. WISP-1 is expressed by fibroblasts and osteoblasts, and may contribute to fracture healing by promoting bone cell formation (1, 10, 11).

  1. Pennica, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14717.
  2. Tanada, S. et al. (2001) Oncogene 20:5525.
  3. Brigstock, D.R. et al. (2003) J. Clin. Pathol. Mol. Pathol. 56:127.
  4. Desnoyers, L. (2004) Curr. Pharm. Des. 10:3913.
  5. Brigstock, D.R. (2003) J. Endocrinol. 178:169.
  6. Hashimoto, Y. et al. (1998) J. Exp. Med. 187:289.
  7. SwissProt Accession # O54775.
  8. SwissProt Accession # Q99PP0.
  9. SwissProt Accession # O95388
  10. French, D.M. et al. (2004) Am. J. Pathol. 165:855.
  11. Parisi, M.S. et al. (2006) Bone 38:671.
Long Name
Wnt-1 Induced Secreted Protein 1
Entrez Gene IDs
8840 (Human); 22402 (Mouse); 102125044 (Cynomolgus Monkey)
Alternate Names
CCN family member 4; CCN4; CCN4WISP1tc; WISP1; WISP-1; WISP1c; WISP1i; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; Wnt-1-induced secreted protein; WNT1-inducible-signaling pathway protein 1

Citations for Recombinant Mouse WISP-1/CCN4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Data-driven learning how oncogenic gene expression locally alters heterocellular networks
    Authors: DJ Klinke Ii, A Fernandez, W Deng, A Razazan, H Latifizade, AC Pirkey
    Nature Communications, 2022-04-13;13(1):1986.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Wisp1 is a circulating factor that stimulates proliferation of adult mouse and human beta cells
    Authors: R Fernandez-, A García-Ala, Y Esteban, J Mir-Coll, B Serra-Nava, M Fontcubert, C Broca, M Armanet, A Wojtuscisz, V Kram, MF Young, J Vidal, R Gomis, R Gasa
    Nat Commun, 2020-11-25;11(1):5982.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells, Whole Tissue
    Applications: Bioassay, Cell Culture, In Vivo
  3. WNT1-inducible signaling pathway protein 1 (WISP1/CCN4) stimulates melanoma invasion and metastasis by promoting the epithelial - mesenchymal transition
    Authors: W Deng, A Fernandez, SL McLaughlin, DJ Klinke
    J. Biol. Chem., 2019-02-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Aging Disrupts Muscle Stem Cell Function by Impairing Matricellular WISP1 Secretion from Fibro-Adipogenic Progenitors
    Authors: L Lukjanenko, S Karaz, P Stuelsatz, U Gurriaran-, J Michaud, G Dammone, F Sizzano, O Mashinchia, S Ancel, E Migliavacc, S Liot, G Jacot, S Metairon, F Raymond, P Descombes, A Palini, B Chazaud, MA Rudnicki, CF Bentzinger, JN Feige
    Cell Stem Cell, 2019-01-24;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
    Authors: Quan Li
    Mol. Med., 2016-01-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay


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Reviews for Recombinant Mouse WISP-1/CCN4 Protein, CF

Average Rating: 4.8 (Based on 5 Reviews)

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Recombinant Mouse WISP-1/CCN4 Protein, CF
By Anonymous on 08/20/2021
Application: CellProlif

Recombinant Mouse WISP-1/CCN4 Protein, CF
By Anonymous on 03/14/2021
Application: CellProlif

Recombinant Mouse WISP-1/CCN4 Protein, CF
By Anonymous on 07/05/2019
Application: CellProlif

Recombinant Mouse WISP-1/CCN4 Protein, CF
By Anonymous on 04/28/2018
Application: Cell migration/motility

Recombinant Mouse WISP-1/CCN4 Protein, CF
By Anonymous on 07/12/2017
Application: CellProlif