Recombinant Rat CD34 Fc Chimera Protein, CF

Name: Recombinant Rat CD34 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 9654-CD
Price: 579 USD
Availability: InStock

R&D Systems | Catalog # 9654-CD

Submit a review

Product Documents

100% Guarantee

Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

R&D Systems NS0-derived Recombinant Rat CD34 Fc Chimera Protein (9654-CD)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_001100672

Structure / Form

Disulfide-linked homodimer

View all CD34 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat CD34 protein

Rat CD34 (Glu37-Leu292) Accession # NP_001100672	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu37

Predicted Molecular Mass

55 kDa

SDS-PAGE

98-111 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9654-CD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS. Loading...
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD34

CD34 is a glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen (1, 2, 3). Rat CD33 cDNA encodes a 386 amino acid (aa) precursor that contains a 36 aa signal sequence, a 256 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, rat CD34 shares 81% and 52% aa sequence identity with mouse and human CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC) (4, 5). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors (6). CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.