Recombinant Rat CTGF Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.|
|Reconstitution||Reconstitute at 500 μg/mL in water.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Recombinant Rat CTGF (Catalog # 9237-CT) supports the adhesion of Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1-5 μg/mL
Connective Tissue Growth Factor (CTGF), also known as CCN2, is a 36-38 kDa member of the CCN (CYR61/CTGF/NOV) family of secreted matricellular proteins (1). Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain (2). Rat CTGF shares 94% and 99% amino acid (aa) sequence identity with human and mouse CTGF, respectively. CTGF promotes cell adhesion through interactions with a range of cell surface molecules including heparan sulfate proteoglycans (HSPG), LRPAP, and Integrins alpha M, alpha V beta 3, alpha 6 beta 1, alpha IIb beta 3 (3-7). It also binds to and regulates signaling through the Wnt receptors LRP6 and Frizzled-8 and the NGF receptors TrkA and NGF R (8, 9). In addition, CTGF binds directly to BMP-4, TGF-beta 1, TGF-beta 2, and VEGF 165 (10, 11). It blocks BMP-4 and VEGF induced responses but enhances TGF-beta induced responses (10-13). Within VEGF complexes, CTGF can be degraded by a variety of proteases, resulting in restoration of angiogenic activity (11). CTGF promotes fibroblast differentiation from mesenchymal stem cells and their production of type I Collagen and Tenascin C (5, 14). It promotes chondrocyte proliferation and cartilage matrix synthesis (15-17). CTGF is expressed in vascular mural and endothelial cells (EC) during development and promotes pericyte-EC association and angiogenesis (2, 17, 18, 19). It is expressed in the cerebral cortex and olfactory bulb and plays an important role in nervous system development (8, 12).
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Citations for Recombinant Rat CTGF Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Structure, signals, and cellular elements of the mouse gastric mesenchymal niche
Authors: E Manieri, G Tie, D Seruggia, S Madha, A Maglieri, K Huang, Y Fujiwara, K Zhang, S Orkin, R He, N McCarthy, RA Shivdasani
bioRxiv : the preprint server for biology, 2023;0(0):.
Sample Types: Organoid
CCN2-induced lymphangiogenesis is mediated by the integrin alphavbeta5-ERK pathway and regulated by DUSP6
Authors: S Hashiguchi, T Tanaka, R Mano, S Kondo, S Kodama
Scientific Reports, 2022;12(1):926.
Sample Types: In Vivo
Applications: In Vivo
Glycocholic acid aggravates liver fibrosis by promoting the up-regulation of connective tissue growth factor in hepatocytes
Authors: Z Yuan, J Wang, H Zhang, Y Chai, Y Xu, Y Miao, Z Yuan, L Zhang, Z Jiang, Q Yu
Cellular Signalling, 2022;101(0):110508.
A 3D-Bioprinted dual growth factor-releasing intervertebral disc scaffold induces nucleus pulposus and annulus fibrosus reconstruction
Authors: B Sun, M Lian, Y Han, X Mo, W Jiang, Z Qiao, K Dai
Bioact Mater, 2021;6(1):179-190.
Sample Types: Whole Cells
Fluvastatin inhibits advanced glycation end products-induced proliferation, migration, and extracellular matrix accumulation in vascular smooth muscle cells by targeting connective tissue growth factor
Authors: AR Hwang, JO Nam, YJ Kang
Korean J. Physiol. Pharmacol., 2018;22(2):193-201.
Sample Types: Whole Cells
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