Recombinant Rat IL-10 Protein, CF

(8 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.12-0.72 ng/mL.
  • Source
    E. coli-derived Ser19-Asn178
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser19
  • Structure / Form
    Noncovalently-linked homodimer
  • Predicted Molecular Mass
    19 kDa (monomer)
  • SDS-PAGE
    16 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
522-RLB/CF
 
522-RLB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Rat IL-10 (Catalog # 522-RLB/CF) promotes MC/9‑2 mouse mast cell proliferation. The ED50 for this effect is 0.12-0.72 ng/mL.
Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL‑19,
IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature rat IL‑10 shares 85% amino acid sequence identity with mouse and 71%-79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8-10). The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12-14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15-17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

  • References:
    1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
    2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
    3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
    4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
    5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
    6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
    7. Hsu, D.-H. et al. (1990) Science 250:830.
    8. Goodman, R. et al. (1992) Biochem. Biophys. Res. Commun. 189:1.
    9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
    10. Syto, R. et al. (1998) Biochemistry 37:16943.
    11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
    12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
    13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
    14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
    15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
    16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
    17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.
  • Long Name:
    Interleukin 10
  • Entrez Gene IDs:
    3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
  • Alternate Names:
    CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Correlation of Gut Microbiota Composition with Resistance to Experimental Autoimmune Encephalomyelitis in Rats
    Front Microbiol, 2016;7(0):2005.
    Species: Rat
    Sample Type: Cell Culture Supernates
    Application: ELISA Standard
  2. Expression and contributions of TRPM7 and KCa2.3/SK3 channels to the increased migration and invasion of microglia in anti-inflammatory activation states.
    Authors: Siddiqui T, Lively S, Ferreira R, Wong R, Schlichter L
    PLoS ONE, 2014;9(8):e106087.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  3. LIMLE, a new molecule over-expressed following activation, is involved in the stimulatory properties of dendritic cells.
    Authors: Le Texier L, Durand J, Lavault A, Hulin P, Collin O, Le Bras Y, Cuturi M, Chiffoleau E
    PLoS ONE, 2014;9(4):e93894.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  4. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.
    Authors: Steelman A, Smith R, Welsh C, Li J
    J Biol Chem, 2014;288(33):23776-87.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  5. Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
    Authors: Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
    Invest. Ophthalmol. Vis. Sci., 2008;49(9):3999-4007.
    Species: Rat
    Sample Type: Aqueous humor
    Application: Bioassay
  6. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
    Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
    Stem Cells, 2008;26(4):1047-55.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: ELISA development
  7. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
    Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
    Crit. Care Med., 2005;33(10):2317-22.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  8. Lipopolysaccharide-induced increase in signalling in hippocampus is abrogated by IL-10--a role for IL-1 beta?
    Authors: Walsh C, Delaney A, Nolan Y, Campbell VA
    J. Neurochem., 2004;88(3):635-46.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo

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