Recombinant Rat IL-2 Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
502-RL-010/CF
502-RL-050/CF
Product Details
Citations (10)
FAQs
Reviews

Recombinant Rat IL-2 Protein, CF Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.04‑0.2 ng/mL.
Source
E. coli-derived rat IL-2 protein
Ala21-Gln155, with and without an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Ala21 & Met
Predicted Molecular Mass
16 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

502-RL/CF

Formulation Supplied as a 0.2 μm filtered solution in Ammonium Acetate and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

502-RL

Formulation Supplied as a 0.2 μm filtered solution in Ammonium Acetate and Glycerol with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
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Background: IL-2

Interleukin-2 (IL-2) is a O-glycosylated four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1 - 3). Mature rat IL-2 shares 66% and 73% amino acid sequence identity with human and mouse IL-2, respectively. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (4 - 6). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma  chain  gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (7). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (8 - 10). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (11, 12).

References
  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. McKnight, A. et al. (1989) Immunogenetics 30:145.
  4. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  5. Wang, X. et al. (2005) Science 310:1159.
  6. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  7. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  8. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  9. Laurence, A. et al. (2007) Immunity 26:371.
  10. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  11. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  12. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.
Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Citations for Recombinant Rat IL-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Dendritic cell-targeted CD40 DNA vaccine suppresses Th17 and ameliorates progression of experimental autoimmune glomerulonephritis
    Authors: Q Li, Q Cao, C Wang, H Nguyen, XM Wang, G Zheng, YM Wang, S Hu, SI Alexander, DCH Harris, Y Wang
    J. Leukoc. Biol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A Crucial Role of CXCL14 for Promoting Regulatory T Cells Activation in Stroke
    Authors: HT Lee, SP Liu, CH Lin, SW Lee, CY Hsu, HK Sytwu, CH Hsieh, WC Shyu
    Theranostics, 2017;7(4):855-875.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Type II Natural Killer T Cells that Recognize Sterol Carrier Protein 2 Are Implicated in Vascular Inflammation in the Rat Model of Systemic Connective Tissue Diseases
    Authors: Akihiro Ishizu
    Am. J. Pathol., 2016;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Probing the biomechanical contribution of the endothelium to lymphocyte migration: diapedesis by the path of least resistance.
    Authors: Martinelli R, Zeiger A, Whitfield M, Sciuto T, Dvorak A, Van Vliet K, Greenwood J, Carman C
    J Cell Sci, 2014;127(0):3720-34.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Expression and contributions of TRPM7 and KCa2.3/SK3 channels to the increased migration and invasion of microglia in anti-inflammatory activation states.
    Authors: Siddiqui T, Lively S, Ferreira R, Wong R, Schlichter L
    PLoS ONE, 2014;9(8):e106087.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Time-dependent effects of striatal interleukin-2 on open field behaviour in rats.
    Authors: Karrenbauer BD, Ho YJ, Ludwig V, Lohn J, Spanagel R, Schwarting RK, Pawlak CR
    J. Neuroimmunol., 2009;208(1):10-8.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  7. Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
    Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
    Cell. Signal., 2009;21(1):151-60.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury.
    Authors: Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH
    Stem Cells, 2008;26(4):1047-55.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Developmet
  9. Molecular identification of lyso-glycerophosphocholines as endogenous immunosuppressives in bovine and rat gonadal fluids.
    Authors: Foulds LM, Boysen RI, Crane M, Yang Y, Muir JA, Smith AI, de Kretser DM, Hearn MT, Hedger MP
    Biol. Reprod., 2008;79(3):525-36.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Autoreactive T cells persist in rats protected against experimental autoimmune encephalomyelitis and can be activated through stimulation of innate immunity.
    Authors: Conant SB, Swanborg RH
    J. Immunol., 2004;172(9):5322-8.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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