Recombinant Rat Integrin alpha V beta 6 Protein, CF

Catalog # Availability Size / Price Qty
9668-AV-050
Recombinant Rat Integrin alpha V beta 6 Protein Binding Activity
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Recombinant Rat Integrin alpha V beta 6 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat Integrin  alpha V beta 6 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human LAP (TGF‑ beta 1) (Catalog # 246-LP) that produces 50% of the optimal binding response is approximately 0.5‑3 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived rat Integrin alpha V beta 6 protein
Rat Integrin alpha V
(Phe31-Val987)
Accession # XP_017456512.1
His-ProGGGSGGGSAcidic Tail HHHHHH
Rat Integrin beta 6
(Gly22-Pro708)
Accession # Q6AYF4
His-ProGGGSGGGSBasic Tail
N-terminusC-terminus
N-terminal Sequence
Analysis
Phe31 (Integrin alpha V) & Gly22 (Integrin beta 6)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
115 kDa (Integrin alpha V) & 83 kDa (Integrin beta 6)
SDS-PAGE
106-153 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9668-AV

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Reconstitution Reconstitute at 500 μg/mL in Water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Rat Integrin alpha V beta 6 Protein Binding Activity View Larger

Immobilized Recombinant Rat Integrin aV beta 6 (Catalog # 9668-AV) at 2.0 µg/mL can bind Recombinant Human LAP (TGF-beta 1) (Catalog # 246-LP) with an ED50 of 0.5-3 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha V beta 6

Integrin  alpha V beta 6 is one of five alpha V integrins and the sole  beta 6 integrin (1, 2). The non-covalent heterodimer of 170 kDa alpha V/CD51 and 95 kDa beta 6 integrin subunits is expressed exclusively on subsets of epithelial cells, especially during development, after injury or inflammation, or on many carcinomas (2-5). The ligand interaction site of alpha V beta 6 is in the N-terminal head region formed by an interaction of the beta 6 vWFA domain with the alpha V beta-propeller structure (2). The alpha V subunit contains domains termed thigh, calf, and calf-2 with a divalent cation-binding site found at a position equivalent to the "knee". The 958 amino acid (aa) rat alpha V ECD, which is cleaved at aa 886 but remains associated, shares 93% and 98% aa sequence identity with human and mouse  alpha V, while the 687 aa rat beta 6 ECD shares 90% and 96% aa sequence identity with human and mouse  beta 6, respectively. Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. The beta 6 C-terminal 11 aa cytoplasmic sequence transduces a signal, enhancing proliferation and inducing MMP-9 expression (6). Either "inside-out" signaling or Mg2+ or Mn2+ binding unfolds and activates the integrin (1). Active alpha V beta 6 binds matrix proteins fibronectin and tenascin C (2). It also binds the TGF-beta latency-associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes (7). This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1 (8, 9). Deletion of beta 6 ablates tonic inhibition of alveolar macrophages by TGF-beta, inhibits intestinal regulatory T cell production, and predisposes mice to inflammatory reactions in the skin, lungs, and intestines where irritations and microbial challenges are frequent (10-12). High alpha V beta 6 expression in carcinomas may contribute to progression through its effects on TGF-beta and MMP activity (3). The foot-and-mouth disease virus and several other viruses can use alpha V beta 6 as a receptor, and soluble alpha V beta 6 may block virus infectivity in vitro (13, 14).

References
  1. Hynes, R.O. (2002) Cell 110:673.
  2. Sheppard, D. (2004) Curr. Opin. Cell Biol. 16:552.
  3. Bandyopadhyay, A. and S. Raghavan (2009) Curr. Drug Targets 10:645.
  4. Wada, J. et al. (1996) J. Cell Biol. 132:1161.
  5. Arend, L.J. et al. (2000) J. Am. Soc. Nephrol. 11:2297.
  6. Dixit, R.B. et al. (1996) J. Biol. Chem. 271:25976.
  7. Munger, J.S. et al. (1999) Cell 96:319.
  8. Fontana, L. et al. (2005) FASEB J. 19:1798.
  9. Jenkins, R.G. et al. (2006) J. Clin. Invest. 116:1606.
  10. Huang, X.Z. et al. (1996) J. Cell Biol. 133:921.
  11. Morris, D.G. et al. (2003) Nature 422:169.
  12. Chen, X. et al. (2011) J. Leukoc. Biol. 90:751.
  13. Berryman, S. et al. (2005) J. Virol. 79:8519.
  14. Heikkila, O. et al. (2009) J. Gen. Virol. 90:197.
Entrez Gene IDs
3685 (Human)
Alternate Names
CD51; Integrin alpha V beta 6; integrin subunit alpha V; ITGAV; MSK8; VNRA; VTNR

FAQs

  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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