Recombinant Rat Kremen-2 Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products
Reviews (2)

Recombinant Rat Kremen-2 Protein, CF Summary

Product Specifications

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. Immobilized rrKremen-2 at 4 µg/mL can bind rhDkk-1 with an apparent KD < 15 nM.
Mouse myeloma cell line, NS0-derived rat Kremen-2 protein
Gly53-Arg395, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
37.5 kDa
45-55 kDa, reducing conditions

Complete Your Experiment

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Kremen-2

Kremen (Kringle-containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains, and an intracellular region with no conserved motifs (1). Two related molecules, Kremen-1 and -2, have been identified. Kremens bind with high affinity to a subset of the secreted Dickkopf proteins (Dkk-1, -2, and -4) to modulate the canonical Wnt signaling pathway (2). This pathway is transduced by a ternary receptor complex composed of Wnt, the seven-transmembrane domain receptor Frizzled, and the LDL-receptor-related protein 5/6 (LRP5/6) coreceptor (3, 4). Dkk-1 and -4 bind directly to the LRP5/6 coreceptor to antagonize the canonical Wnt/ beta -catenin signaling pathway. The planar cell polarity (PCP) signaling pathway is not affected as it does not involve LRP5/6 (2). In contrast, Dkk-3 has no effect on Wnt signaling. Dkk-2 can function either as an LRP agonist or antagonist, depending on whether or not the cell expresses Kremen (5). Kremen cooperates with Dkk to antagonize Wnt signaling via formation of a Kremen-Dkk-LRP ternary complex which is internalized and cleared from the cell surface (4). Binding requires all three extracellular domains of Kremen, and the second cysteine-rich domain of Dkk (4). The predicted rat Kremen-2 cDNA encodes a 490 amino acid (aa) glycosylated protein with a 51 aa signal peptide, a 340 aa extracellular domain, a 24 aa transmembrane domain, and a 75 aa cytoplasmic domain. Within the ECD, rat Kremen-2 shares 91% and 98% aa sequence identity with human and mouse Kremen-2, respectively, and 46% with rat Kremen-1.

  1. Nakamura, T. et al. (2001) Biochim. Biophys. Acta 1518:63.
  2. Niehrs, C. (2006) Oncogene 25:7469.
  3. Davidson G. et al. (2002) Development 129:5587.
  4. Mao, B. et al. (2002) Nature 417:664.
  5. Mao, B. and C. Niehrs (2003) Gene 302:179.
Long Name
Kringle-containing Transmembrane Protein 2
Entrez Gene IDs
79412 (Human); 73016 (Mouse)
Alternate Names
Dickkopf receptor 2; kremen protein 2; Kremen2; Kremen-2; kringle containing transmembrane protein 2; Kringle domain-containing transmembrane protein 2; KRM2Kringle-containing protein marking the eye and the nose; MGC10791; MGC16709


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Rat Kremen-2 Protein, CF

Average Rating: 4.5 (Based on 2 Reviews)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Recombinant Rat Kremen-2 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Recombinant Rat Kremen-2 Protein, CF
By Gerre Lafond on 02/19/2021
Application: Binding assay/Protein-protein interaction

Recombinant Rat Kremen-2 Protein, CF
By Anonymous on 10/15/2020
Application: SDS-PAGE Control