Recombinant Rat Leptin Protein, CF

Catalog # Availability Size / Price Qty
598-LP-01M
598-LP-05M

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Recombinant Rat Leptin Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human Leptin R. The ED50 for this effect is 0.05-0.3 ng/mL.
Source
E. coli-derived rat Leptin/OB protein
Val22-Cys167, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
16.2 kDa

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598-LP

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

598-LP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCI, pH 8.0.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Leptin/OB

Leptin is a identified protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism, and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. Rat Leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. Rat Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

References
  1. Considine, R. and Caro, J. (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al., (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Entrez Gene IDs
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

Citations for Recombinant Rat Leptin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. The Acute Effects of Leptin on the Contractility of Isolated Rat Atrial and Ventricular Cardiomyocytes
    Authors: A Khokhlova, T Myachina, X Butova, A Kochurova, E Polyakova, M Galagudza, O Solovyova, G Kopylova, D Shchepkin
    International Journal of Molecular Sciences, 2022;23(15):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Modulation of Sirt1 and FoxO1 on Hypothalamic Leptin-Mediated Sympathetic Activation and Inflammation in Diet-Induced Obese Rats
    Authors: X Liu, H Zheng
    Journal of the American Heart Association, 2021;10(14):e020667.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  3. Hyperleptinemia results in systemic inflammation and the exacerbation of ischemia-reperfusion myocardial injury
    Authors: EA Polyakova, EN Mikhaylov, MM Galagudza, EV Shlyakhto
    Heliyon, 2021;7(11):e08491.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay
  4. Roux-en-Y gastric bypass contributes to weight loss-independent improvement in hypothalamic inflammation and leptin sensitivity through gut-microglia-neuron-crosstalk
    Authors: J Chen, N Haase, SB Haange, R Sucher, J Münzker, E Jäger, K Schischke, F Seyfried, M von Bergen, MK Hankir, U Krügel, WK Fenske
    Molecular Metabolism, 2021;0(0):101214.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay
  5. Responsiveness of hypothalamo-pituitary-adrenal axis to leptin is impaired in diet-induced obese rats
    Authors: AC Shin, SMJ MohanKumar, P Balasubram, MP Sirivelu, K Linning, A Woolcock, M James, PS MohanKumar
    Nutr Diabetes, 2019;9(1):10.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  6. Leptin Aggravates Reflux Esophagitis by Increasing Tissue Levels of Macrophage Migration Inhibitory Factor in Rats
    Authors: T Murata, K Asanuma, N Ara, K Iijima, W Hatta, S Hamada, N Asano, T Koike, A Imatani, A Masamune, T Shimosegaw
    Tohoku J. Exp. Med., 2018;245(1):45-53.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  7. Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution
    Authors: RBS Harris
    Appetite, 2018;132(0):114-121.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. Leptin regulates disc cartilage endplate degeneration and ossification through activation of the MAPK-ERK signalling pathway in�vivo and in�vitro
    Authors: YC Han, B Ma, S Guo, M Yang, LJ Li, SJ Wang, J Tan
    J. Cell. Mol. Med., 2018;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  9. Leptin enhances hypothalamic LDHA-dependent glucose sensing to lower glucose production in high-fat fed rats
    Authors: MA Abraham, M Rasti, PV Bauer, TKT Lam
    J. Biol. Chem., 2018;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  10. Gestational hypoxia disrupts the neonatal leptin surge and programs hyperphagia and obesity in male offspring in the Sprague-Dawley rat
    Authors: VE Vargas, S Gurung, B Grant, K Hyatt, K Singleton, SM Myers, D Saunders, C Njoku, R Towner, DA Myers
    PLoS ONE, 2017;12(9):e0185272.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  11. Leptin-induced basal Akt phosphorylation and its implication in exercise-mediated improvement of insulin sensitivity
    Authors: X Zheng, S Niu
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Leptin Attenuates the Contractile Function of Adult Rat Cardiomyocytes Involved in Oxidative Stress and Autophagy
    Authors: Liu-Jin Luo
    Acta Cardiol Sin, 2016;32(6):723-730.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Fourth ventricle leptin infusions dose dependently activate hypothalamic signal transducer and activator of transcription 3 (STAT3)
    Authors: Bhavna N Desai
    Am. J. Physiol. Endocrinol. Metab., 2016;0(0):ajpendo.00343.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  14. Renal hemodynamic and morphological changes after 7 and 28 days of leptin treatment: the participation of angiotensin II via the AT1 receptor.
    Authors: Thieme K, Oliveira-Souza M
    PLoS ONE, 2015;10(3):e0122265.
    Species: Rat
    Sample Types: In Vivo
  15. Leptin-induced endothelium-dependent vasorelaxation of peripheral arteries in lean and obese rats: role of nitric oxide and hydrogen sulfide.
    Authors: Jamroz-Wisniewska A, Gertler A, Solomon G, Wood M, Whiteman M, Beltowski J
    PLoS ONE, 2014;9(1):e86744.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  16. Blockade of the cerebral aqueduct in rats provides evidence of antagonistic leptin responses in the forebrain and hindbrain.
    Authors: Vaill M, Desai B, Harris R
    Am J Physiol Endocrinol Metab, 2014;306(4):E414-23.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  17. Leptin augments the acute suppressive effects of insulin on hepatic very low-density lipoprotein production in rats.
    Authors: Huang W, Metlakunta A, Dedousis N, Ortmeyer HK, Stefanovic-Racic M, O'Doherty RM
    Endocrinology, 2009;150(5):2169-74.
  18. Leptin increases adult hippocampal neurogenesis in vivo and in vitro.
    Authors: Garza JC, Guo M, Zhang W, Lu XY
    J. Biol. Chem., 2008;283(26):18238-47.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  19. Leptin modulates the response to oleic acid in the pharynx.
    Authors: Kitagawa J, Shingai T, Kajii Y, Takahashi Y, Taguchi Y, Matsumoto S
    Neurosci. Lett., 2007;423(2):109-12.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  20. Augmenting leptin circadian rhythm following a weight reduction in diet-induced obese rats: short- and long-term effects.
    Authors: Buison A, Pellizzon M, Ordiz F, Jen KL
    Metab. Clin. Exp., 2004;53(6):782-9.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  21. BiaCore analysis of leptin-leptin receptor interaction: evidence for 1:1 stoichiometry.
    Authors: Mistrik P, Moreau F, Allen JM
    Anal. Biochem., 2004;327(2):271-7.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  22. Changes in hypothalamic expression levels of galanin-like peptide in rat and mouse models support that it is a leptin-target peptide.
    Authors: Kumano S, Matsumoto H, Takatsu Y, Noguchi J, Kitada C, Ohtaki T
    Endocrinology, 2003;144(6):2634-43.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Recombinant Rat Leptin Protein, CF
By Anonymous on 08/12/2016
Application: In vivo study