Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF

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Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 400 ng on a nitrocellulose-coated microplate.
Mouse myeloma cell line, NS0-derived rat MAG/Siglec-4a protein
Accession # P07722
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
81 kDa (monomer)
120 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: MAG/Siglec-4a

MAG (Myelin-Associated Glycoprotein), a type I transmembrane glycoprotein containing five Ig-like domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell-surface glycan containing sialic acid residues define the I-type sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal re-growth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury.

  1. Kelm, S. et al. (1994) Current Biology 4:965.
  2. McKerracher, L. et al. (1994) Neuron 13:805.
  3. Tang, S. et al. (1997) Molecular and Cellular Neuroscience 9:333.
  4. Cai, D. et al. (1999) Neuron 22:89.
Long Name
Myelin-associated Glycoprotein
Entrez Gene IDs
4099 (Human); 17136 (Mouse); 29409 (Rat)
Alternate Names
GMAsialic acid binding Ig-like lectin 4A; MAG; myelin associated glycoprotein; myelin-associated glycoprotein; sialic acid-binding immunoglobulin-like lectin 4A; Siglec4a; Siglec-4a; S-MAG

Citations for Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Myelin-associated glycoprotein combined with chitin conduit inhibits painful neuroma formation after sciatic nerve transection
    Authors: W Pi, C Li, M Zhang, W Zhang, PX Zhang
    Neural regeneration research, 2022;17(6):1343-1347.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  2. MAG induces apoptosis in cerebellar granule neurons through p75NTR demarcating granule layer/white matter boundary
    Authors: D Fernández-, FA Krapacher, A Andersson, CF Ibáñez, L Kisiswa
    Cell Death Dis, 2019;10(10):732.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. miR-155-5p Promotes Dorsal Root Ganglion Neuron Axonal Growth in an Inhibitory Microenvironment via the cAMP/PKA Pathway
    Authors: T Wang, B Li, Z Wang, X Yuan, C Chen, Y Zhang, Z Xia, X Wang, M Yu, W Tao, L Zhang, X Wang, Z Zhang, X Guo, G Ning, S Feng, X Chen
    Int. J. Biol. Sci., 2019;15(7):1557-1570.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Inhibition of neurite outgrowth using commercial myelin associated glycoprotein-Fc in neuro-2a cells
    Authors: F Liu, ML Gao, J Bai, YF Wang, XQ Li
    Neural Regen Res, 2018;13(11):1893-1899.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. ?-Tubulin Acetyltransferase Is a Novel Target Mediating Neurite Growth Inhibitory Effects of Chondroitin Sulfate Proteoglycans and Myelin-Associated Glycoprotein
    Authors: VSC Wong, C Picci, M Swift, M Levinson, D Willis, B Langley
    eNeuro, 2018;5(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. The soluble form of LOTUS inhibits Nogo receptor-mediated signaling by interfering with the interaction between Nogo receptor type 1 and p75 neurotrophin receptor
    Authors: Y Kawakami, Y Kurihara, Y Saito, Y Fujita, T Yamashita, K Takei
    J. Neurosci., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Complement protein C1q modulates neurite outgrowth in vitro and spinal cord axon regeneration in vivo.
    Authors: Peterson S, Nguyen H, Mendez O, Anderson A
    J Neurosci, 2015;35(10):4332-49.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. LOTUS suppresses axon growth inhibition by blocking interaction between Nogo receptor-1 and all four types of its ligand.
    Authors: Kurihara Y, Iketani M, Ito H, Nishiyama K, Sakakibara Y, Goshima Y, Takei K
    Mol Cell Neurosci, 2014;61(0):211-8.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Differential but competitive binding of Nogo protein and class i major histocompatibility complex (MHCI) to the PIR-B ectodomain provides an inhibition of cells.
    Authors: Matsushita H, Endo S, Kobayashi E, Sakamoto Y, Kobayashi K, Kitaguchi K, Kuroki K, Soderhall A, Maenaka K, Nakamura A, Strittmatter SM, Takai T
    J. Biol. Chem., 2011;286(29):25739-47.
    Species: Mouse
    Sample Types: Protein
    Applications: Surface Plasmon Resonance
  10. Bidirectional remodeling of beta1-integrin adhesions during chemotropic regulation of nerve growth.
    Authors: Carlstrom LP, Hines JH, Henle SJ, Henley JR
    BMC Biol., 2011;9(0):82.
    Species: Xenopus
    Sample Types: Whole Cells
  11. Molecular basis of the interactions of the Nogo-66 receptor and its homolog NgR2 with myelin-associated glycoprotein: development of NgROMNI-Fc, a novel antagonist of CNS myelin inhibition.
    Authors: Robak LA, Venkatesh K, Lee H, Raiker SJ, Duan Y, Lee-Osbourne J, Hofer T, Mage RG, Rader C, Giger RJ
    J. Neurosci., 2009;29(18):5768-83.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Synaptic function for the Nogo-66 receptor NgR1: regulation of dendritic spine morphology and activity-dependent synaptic strength.
    Authors: Lee H, Raiker SJ, Venkatesh K, Geary R, Robak LA, Zhang Y, Yeh HH, Shrager P, Giger RJ
    J. Neurosci., 2008;28(11):2753-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Ganglioside inhibition of neurite outgrowth requires Nogo receptor function: identification of interaction sites and development of novel antagonists.
    Authors: Williams G, Wood A, Williams EJ, Gao Y, Mercado ML, Katz A, Joseph-McCarthy D, Bates B, Ling HP, Aulabaugh A, Zaccardi J, Xie Y, Pangalos MN, Walsh FS, Doherty P
    J. Biol. Chem., 2008;283(24):16641-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway.
    Authors: Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H
    Biochem. Biophys. Res. Commun., 2007;365(2):375-80.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A.
    Authors: Wright KT, El Masri W, Osman A, Roberts S, Chamberlain G, Ashton BA, Johnson WE
    Biochem. Biophys. Res. Commun., 2007;354(2):559-66.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Cleavage of myelin associated glycoprotein by matrix metalloproteinases.
    Authors: Milward E, Kim KJ, Szklarczyk A, Nguyen T, Melli G, Nayak M, Deshpande D, Fitzsimmons C, Hoke A, Kerr D, Griffin JW, Calabresi PA, Conant K
    J. Neuroimmunol., 2007;193(1):140-8.
    Species: N/A
    Sample Types: N/A
    Applications: Enzyme Assay Substrate
  17. Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs.
    Authors: Willis DE, van Niekerk EA, Sasaki Y, Mesngon M, Merianda TT, Williams GG, Kendall M, Smith DS, Bassell GJ, Twiss JL
    J. Cell Biol., 2007;178(6):965-80.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  18. Promotion of axon regeneration by myelin-associated glycoprotein and Nogo through divergent signals downstream of Gi/G.
    Authors: Hasegawa Y, Fujitani M, Hata K, Tohyama M, Yamagishi S, Yamashita T
    J. Neurosci., 2004;24(30):6826-32.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay
  19. A p75(NTR) and Nogo receptor complex mediates repulsive signaling by myelin-associated glycoprotein.
    Authors: Wong ST, Henley JR, Kanning KC, Huang KH, Bothwell M, Poo MM
    Nat. Neurosci., 2002;5(12):1302-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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Recombinant Rat MAG/Siglec-4a Fc Chimera Protein, CF
By Anonymous on 08/18/2017
Application: Binding assay/Protein-protein interaction