Recombinant Rat MIS RII Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1618-MR-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Rat MIS RII Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhMIS at 3 µg/mL (100 µL/well) can bind rrMIS RII/Fc Chimera with a linear range of 1.6-100 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (stably transfected)-derived rat MIS RII protein
Rat MIS RII
(Pro19 - Pro144)
Accession # Q62893
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Pro19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40 kDa (monomer)
SDS-PAGE
50-55 kDa, reducing conditions

Complete Your Experiment

Product Datasheets

You must select a language.

x

1618-MR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1618-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: MIS RII

Müllerian inhibiting substance (MIS), also named anti-Müllerian hormone (AMH) is a tissue-specific TGF-beta superfamily growth factor. Its expression is restricted to fetal testis, plus postnatal testis and ovary (1). MIS induces Mullerian duct (female reproductive tract) regression during sexual differentiation in the male embryo and has been shown to have a regulatory role in gonads postnatally (1). Like other TGF-beta superfamily members, MIS signals via a heteromeric receptor complex consisting of a type I and a type II receptor serine/threonine kinase. Depending on the cell context, different type I receptors (including Act RIA/ALK2, BMP RIA/ALK3, and BMP RIB/ALK6) that are shared by other TGF-beta superfamily members, can be utilized for MIS signaling (1). In contrast, the type II MIS receptor (MIS RII) is unique and does not bind other TGF-beta superfamily members (1, 2). Upon ligand binding, MIS RII recruits the non-ligand binding type I receptor into the complex, resulting in phosphorylation the BMP-like signaling pathway effector proteins Smad1, Smad5 and Smad 8 (1).

The gene for rat MIS RII was isolated separately by two groups working from Sertoli cell and fetal ovary cDNA libraries (3, 4). MIS RII comprises a 557 amino acid (aa) residue type I transmembrane protein with a putative 17 aa signal peptide. Mature MIS RII has a 127 aa cysteine-rich extracellular domain containing 2 potential N-glycosylation sites, a 21 aa transmembrane domain, and a 392 aa cytoplasmic region with a serine/threonine kinase domain (3, 4). Rat MIS RII shares 95% and 82% aa sequence identity with the mouse and human homologues, respectively (5). MIS RII is expressed in the mesenchymal cells surrounding the Mullerian ducts during embryonic development. Postnatally, it is expressed in uterine tissues and rodent Leydig cells, and coexpressed with MIS in the testicular Sertoli and ovarian granulosa cells (1, 6). The expression of MIS RII in the Mullerian mesenchyme is regulated by Wnt7a signaling from nearby epithelium through the canonical Wnt pathway. Wnt7a mutant mice do not express MIS RII, and do not experience Mullerian duct regression (7).

References
  1. Josso, N and N. diClemente (2003) Trends Endo. Met. 14:91.
  2. Mishna, Y. et al. (1999) Endocrinology 140:2084.
  3. Baarends, W. et al. (1994) Development 120:189.
  4. di Clement, N. et al. (1994) Mol. Endocrinol. 8:1006.
  5. Mishina, Y. et al. (1997) Biochem. Biophys. Res. Comm. 237:741.
  6. Teixeira, J. et al. (1996) Endocrinology 137:160.
  7. Hossain, A. and G. Saunders (2003) J. Biol. Chem. 278:26511.
Long Name
Mullerian-Inhibiting Substance Type II Receptor
Entrez Gene IDs
269 (Human); 110542 (Mouse); 29530 (Rat)
Alternate Names
AMH type II receptor; AMHR2; AMHREC 2.7.11.30; AMHRII; C14; MIS RII; MISR2; MISRII; MISRIIMIS type II receptor; MRII; Muellerian hormone type II receptor; Muellerian hormone type-2 receptor; Mullerian hormone receptor, type II; Mullerian inhibiting substance type II receptor

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Rat MIS RII Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Rat MIS RII Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Rat MIS RII Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review