Recombinant Rat Nectin-4 His-tag Protein, CF Summary
Tyr28-Val348, with a C-terminal 6-His tag
Immobilized Recombinant Rat Nectin‑4 (Catalog #9997-N4) supports the adhesion of NIH‑3T3 mouse embryonic fibroblastcells. The ED50 for this effect is 0.1‑0.8 μg/mL.
2 μg/lane of Recombinant Rat Nectin‑4 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 38-48 kDa.
Nectin-4, also known as Poliovirus receptor-related protein 4 (Gene name: PVRL4), is a 66-kDa type I transmembrane glycoprotein belonging to the Nectin immunoglobulin superfamily (1). The Latin word necto means "to connect", indicating the role of nectins in Ca2+‑independent cell-cell adhesion (2). Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4 (1‑3). Human Nectin-4 is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain (1, 4, 5). In many human ductal breast or non-small cell lung carcinomas, Nectin‑4 is up-regulated and a soluble 43-kDa form is found in the plasma (4-6). This form is generated from the membrane protein via the action of TACE/ADAM-17 (6). Nectin extracellular domains (ECDs) contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type (1, 3). Within the ECD, rat Nectin-4 shares 91%, and 97% amino acid sequence identity with human and mouse Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins (1, 2). These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections (2, 7). In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development (7). High Nectin-4/PVRL4 expression was associated with poor-prognosis in some invasive breast cancers and may be a new promising prognostic biomarker and specific therapeutic target (8).
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