Recombinant Rat Neurexin 1 alpha Protein, CF

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4485-NX-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Rat Neurexin 1 alpha Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rrNRXN-1a at 0.5 µg/mL (100 µL/well) can bind biotinylated rrNXPH-1 with a linear range of 0.03‑2 µg/mL.
Source
Mouse myeloma cell line, NS0-derived rat Neurexin 1 alpha/NRXN1a protein
Leu31-Thr1431 (His1137Tyr), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu31
Predicted Molecular Mass
154.7 kDa
SDS-PAGE
165-175 kDa, reducing conditions

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4485-NX

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4485-NX

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Neurexin 1 alpha/NRXN1a

Neurexin 1, also known as NRXN1a, is a neuronal type I transmembrane molecule that is encoded by one of three Neurexin genes (1, 2). Mature rat Neurexin 1 alpha is a 130 - 200 kDa N- and O-glycosylated protein with a 1424 amino acid (aa) extracellular domain (ECD) and a 55 aa cytoplasmic region. The ECD contains six LNS domains interspersed with three EGF-like domains and a juxtamembrane O-glycosylation region; the cytoplasmic domain contains a PDZ recognition motif (1, 2). The ECD of rat Neurexin 1 alpha shares 92% and 97% aa sequence identity with human and mouse Neurexin 1 alpha, respectively. It shares 68% aa sequence identity with rat Neurexin 2 alpha and 3 alpha. Transcription of the Neurexin 1 gene from an internal promoter gives rise to Neurexin 1 beta which lacks the first five LNS domains and all of the EGF-like domains (3). Neurexins are combinatorially spliced at five canonical sites, giving rise to over 1000 potential isoforms (3, 4). This recombinant protein corresponds to the ECD of SwissProt # Q63372, isoform 13, which has short deletions at splice sites 1 and 2. Neurexin 1 alpha is primarily expressed in the brain where it is localized to presynaptic terminals (3, 5). It binds black widow spider toxin alpha -latrotoxin, plus dystroglycan, neurexophilin 1 and 3, and the postsynaptic neuroligins (6 - 9). The interaction between Neurexins and neuroligins is complex and is restricted to particular combinations of splice forms of each binding partner (9). These interactions promote the differentiation and maintenance of postsynaptic GABA and NMDA, but not AMPA terminals (10, 11). At presynaptic terminals, as well as in pituitary melanotrophs, alpha Neurexins are required for coupling voltage-dependent calcium channel signaling to neurotransmitter or hormone release (12 - 14).

References
  1. Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.
  2. Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833. 
  3. Ushkaryov, Y.A. et al. (1992) Science 257:50. 
  4. Rowen, L. et al. (2002) Genomics 79:587. 
  5. Dean, C. et al. (2003) Nat. Neurosci. 7:708. 
  6. Geppert, M. et al. (1998) J. Biol. Chem. 273:1705. 
  7. Sugita, S. et al. (2001) J. Cell Biol. 154:435. 
  8. Missler, M. et al. (1998) J. Biol. Chem. 273:34716.
  9. Boucard, A.A. et al. (2005) Neuron 48:229.
  10. Chih, B. et al. (2006) Neuron 51:171.
  11. Kattenstroth, G. et al. (2004) Proc. Natl. Acad. Sci. 101:2607.
  12. Missler, M. et al. (2003) Nature 423:939.
  13. Zhang, W. et al. (2005) J. Neurosci. 25:4330.
  14. Dudanova, I. et al. (2006) J. Neurosci. 26:10599.
Entrez Gene IDs
9378 (Human); 18189 (Mouse); 60391 (Rat)
Alternate Names
alpha-Latrotoxin Receptor; Neurexin 1 alpha; NRXN1; NRXN1a

Citations for Recombinant Rat Neurexin 1 alpha Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Interaction of A? Oligomers with Neurexin 2? and Neuroligin 1 Mediates Synapse Damage and Memory Loss in Mice
    Authors: J Brito-More, MV Lourenco, MM Oliveira, JF Vital, FC Ribeiro, JH Ledo, LP Diniz, MH Magdesian, HM Melo, F Barros-Ara, JM de Souza, SV Alves-Leon, FC Gomes, JR Clarke, CP Figueiredo, FG De Felice, ST Ferreira
    J. Biol. Chem, 2017;0(0):.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  2. Neurexophilin 1 suppresses the proliferation of hematopoietic progenitor cells.
    Authors: Kinzfogl J, Hangoc G, Broxmeyer HE
    Blood, 2011;118(3):565-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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